ORBi: Leyh-Bouille Mélina - The Streptomyces K15 Dd-Peptidase/Penicillin-Binding Protein. Active Site and Sequence of the N-Terminal Region

Reference : The Streptomyces K15 Dd-Peptidase/Penicillin-Binding Protein. Active Site and Sequence o...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/99256

Title :	The Streptomyces K15 Dd-Peptidase/Penicillin-Binding Protein. Active Site and Sequence of the N-Terminal Region
Language :	English
Author, co-author :	Leyh-Bouille, Mélina [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
	Van Beeumen, Jozef [Université de Liège - ULg > Rijksuniversiteit-Gent > Laboratorium voor Microbiologie > > >]
	Renier-Pirlot, Suzanne [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
	Joris, Bernard [Université de Liège - ULg > Institut de Chimie > > Service de Microbiologie > >]
	Distèche, Martine [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
	Ghuysen, Jean-Marie [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > > >]
Publication date :	1-Jun-1989
Journal title :	Biochemical Journal
Publisher :	Portland Press
Volume :	260
Pages :	601-604
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0264-6021
e-ISSN :	1470-8728
City :	London
Country :	United Kingdom
Keywords :	[en] Peptidase ; Bacteria ; Actinomycetes ; Actinomycetales ; Streptomycetaceae ; Enzyme ; Streptomyces ; Column chromatography ; Radiolabelling ; N terminal-Sequence ; Active site ; Penicillin ; Binding protein
	[fr] Peptidase ; Bactérie ; Actinomycetales ; Streptomycetaceae ; Streptomyces ; Chromatographie colonne ; Marquage radioisotopique ; Séquence N terminale ; Site actif ; Pénicilline ; Protéine de liaison
	[es] Enzima ; Streptomycetaceae ; Streptomyces ; Cromatografía columna ; Marcación radioisotópica ; Secuencia N terminal ; Lugar activo ; Proteina de enlace
Abstract :	[en] The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high homology with that of other penicillin-interactive proteins or domains. The active-site serine residue of the conserved tetrad Ser-Xaa-Xaa-Lys occurs at position 35. There is no indication for the presence of a signal peptide or an N-terminal hydrophobic sequence, suggesting that the Streptomyces K15 enzyme is probably anchored to the membrane by a C-terminal peptide segment.
Funders :	Fonds de la Recherche Scientifique Médicale - FRSM ; Fond de la Recherche de la Faculté de Médecine ULg
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/99256

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