ORBi: Coupienne Isabelle - RIP3 Expression Induces a Death Proﬁle Change in U2OS Osteosarcoma Cells After 5-ALA-PDT

Reference : RIP3 Expression Induces a Death Proﬁle Change in U2OS Osteosarcoma Cells After 5-ALA-PDT


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/99058

Title :	RIP3 Expression Induces a Death Proﬁle Change in U2OS Osteosarcoma Cells After 5-ALA-PDT
Language :	English
Author, co-author :	Coupienne, Isabelle [Université de Liège - ULg > > GIGA-R : Virologie - Immunologie >]
	Fettweis, Grégory [Université de Liège - ULg > > > GIGA - Utilisateurs machines]
	Piette, Jacques [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie >]
Publication date :	Sep-2011
Journal title :	Lasers in Surgery and Medicine
Publisher :	Wiley Liss, Inc.
Volume :	43
Issue/season :	7
Pages :	557–564
Audience :	International
ISSN :	0196-8092
e-ISSN :	1096-9101
City :	New York
Country :	NY
Keywords :	[en] photodynamic therapy ; osteosarcoma ; RIP3
Abstract :	[en] Background and Objective The receptor-interacting protein 3 (RIP3) has recently been outlined as a key necrosis mediator but is also thought to participate in the regulation of apoptosis. The aim of this study is to compare the cell death profile induced by 5-aminolevulic acid (5-ALA)-mediated photodynamic therapy (PDT) in the RIP3-deficient cell line U2OS and in U2OS cells in which the expression of RIP3 was restored. Materials and Methods RIP3-expressing U2OS cells (RIP3-U2OS) were obtained after transfection and antibiotic selection. Wild type and RIP3-U2OS cells were treated by 5-ALA-PDT. Overall cell viability was evaluated and different parameters characteristic of apoptosis, autophagy, and necrosis were studied. Results Surprisingly, the survival of RIP3-U2OS cells was higher compared to that of the wild type cells. In addition, RIP3-U2OS cell death was decreased by a zVAD-fmk pre-treatment. A higher cleavage of caspase-3, 7, 8, 9, and PARP was also detected in these cells, pointing out to the activation of caspase-dependent apoptosis. In parallel, a thrust of autophagy was clearly identified in the RIP3-U2OS cells. Conversely, RIP3-U2OS exhibited a lower level of necrosis than the wild types. Interestingly, necrostatin-1 efficiently decreased necrosis level in RIP3-U2OS but not in wild type cells. Conclusion Expression of RIP3 in U2OS cells led to a better survival but also to a death profile change in response to PDT. The apoptotic and autophagic pathways were clearly up-regulated compared to the RIP3-deficient wild type cells. However, induction of necrosis was weaker in the RIP3-U2OS cells. In this context, autophagy is likely to play a protective role against PDT-induced cell death and to allow a better survival of RIP3-U2OS cells. This work also highlights the important role played by RIP3 in the apoptotic pathway, although the modalities are still widely unknown.
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Permalink :	http://hdl.handle.net/2268/99058

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	10.1002_lsm.21088.pdf		Author postprint	487.45 kB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5