ORBi: Tsumbu César Ndele - Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes

Reference : Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leave...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health Life sciences : Food science Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/99044

Title :	Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
Language :	English
Alternative title :	[fr] Activités antioxydantes et antiradicalaires des feuilles de Manihot esculenta (Crantz) et d'autres légumes verts tropicaux sélectionnés, investiguées sur lipoperoxydation et des monocytes activés au phorbol 12-myristate, 13-acétate (PMA)
Author, co-author :	Tsumbu, César Ndele [Université de Liège - ULg > > > Form. doc. sc. bioméd. & pharma.]
	Deby-Dupont, Ginette [Université de Liège - ULg > > Centre de l'Oxygène, Recherche et Développement, CORD > >]
	Tits, Monique [Université de Liège - ULg > Département de pharmacie > Phytochimie et phytothérapie >]
	Angenot, Luc [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
	Franck, Thierry [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Serteyn, Didier [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Publication date :	16-Sep-2011
Journal title :	Nutrients
Publisher :	Molecular Diversity Preservation International (MDPI)
Volume :	3
Issue/season :	9
Pages :	818-838
Audience :	International
ISSN :	2072-6643
City :	Basel
Country :	Switzerland
Keywords :	[en] monocytes ; lipid peroxidation ; reactive oxygen species ; green vegetable ; antioxidant ; polyphenols
Abstract :	[en] Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by migrants from sub-Saharan Africa living in Western Europe and by the people in the origin countries, where these plants are also used in the folk medicine. Manihot leaves are also eaten in Latin America and some Asian countries. This work investigated the capacity of aqueous extracts prepared from those vegetables to inhibit the peroxidation of a linoleic acid emulsion. Short chain, volatile C-compounds as markers of advanced lipid peroxidation were measured by gas chromatography by following the ethylene production. The generation of lipid hydroperoxides, was monitored by spectroscopy using N-N′-dimethyl-p-phenylene-diamine (DMPD). The formation of intermediate peroxyl, and other free radicals, at the initiation of the lipid peroxidation was investigated by electron spin resonance, using α-(4-pyridyl-1-oxide)-N-tert-butylnitrone as spin trap agent. The ability of the extracts to decrease the cellular production of reactive oxygen species (ROS) in “inflammation like” conditions was studied by fluorescence technique using 2′,7′-dichlorofluorescine-diacetate as fluorogenic probe, in a cell model of human monocytes (HL-60 cells) activated with phorbol ester. Overall the extracts displayed efficient concentration-dependent inhibitory effects. Their total polyphenol and flavonoid content was determined by classic colorimetric methods. An HPLC-UV/DAD analysis has clearly identified the presence of some polyphenolic compounds, which explains at least partially the inhibitions observed in our models. The role of these plants in the folk medicine by sub-Saharan peoples as well as in the prevention of oxidative stress and ROS related diseases requires further consideration.
Research units :	Faculté de médecine; département de pharmacie, CIRM, laboratoire de pharmacognosie/ Faculté des sciences, centre de l'oxygène, recherche et développement (CORD)
Funders :	1) SP Partnership Association Lich (Germany), 2) Biebergemünder Association for South Development” (Germany), 3) International Help Service IPHD Linz (Austria)
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/99044
	also: http://hdl.handle.net/2268/112629
DOI :	10.3390/nu3090818

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