Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
monocytes; lipid peroxidation; reactive oxygen species; green vegetable; antioxidant; polyphenols
Abstract :
[en] Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by migrants from sub-Saharan Africa living in Western Europe and by the people in the origin countries, where these plants are also used in the folk medicine. Manihot leaves are also eaten in Latin America and some Asian countries. This work investigated the capacity of aqueous extracts prepared from those vegetables to inhibit the peroxidation of a linoleic acid emulsion. Short chain, volatile C-compounds as markers of advanced lipid peroxidation were measured by gas chromatography by following the ethylene production. The generation of lipid hydroperoxides, was monitored by spectroscopy using N-N′-dimethyl-p-phenylene-diamine (DMPD). The formation of intermediate peroxyl, and other free radicals, at the initiation of the lipid peroxidation was investigated by electron spin resonance, using α-(4-pyridyl-1-oxide)-N-tert-butylnitrone as spin trap agent. The ability of the extracts to decrease the cellular production of reactive oxygen species (ROS) in “inflammation like” conditions was studied by fluorescence technique using 2′,7′-dichlorofluorescine-diacetate as fluorogenic probe, in a cell model of human monocytes (HL-60 cells) activated with phorbol ester. Overall the extracts displayed efficient concentration-dependent inhibitory effects. Their total polyphenol and flavonoid content was determined by classic colorimetric methods. An HPLC-UV/DAD analysis has clearly identified the presence of some polyphenolic compounds, which explains at least partially the inhibitions observed in our models. The role of these plants in the folk medicine by sub-Saharan peoples as well as in the prevention of oxidative stress and ROS related diseases requires further consideration.
Research center :
Faculté de médecine; département de pharmacie, CIRM, laboratoire de pharmacognosie/ Faculté des sciences, centre de l'oxygène, recherche et développement (CORD)
Disciplines :
Food science Veterinary medicine & animal health Biochemistry, biophysics & molecular biology
Deby-Dupont, Ginette; Université de Liège - ULiège > Centre de l'Oxygène, Recherche et Développement, CORD
Tits, Monique ; Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Language :
English
Title :
Antioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
Alternative titles :
[fr] Activités antioxydantes et antiradicalaires des feuilles de Manihot esculenta (Crantz) et d'autres légumes verts tropicaux sélectionnés, investiguées sur lipoperoxydation et des monocytes activés au phorbol 12-myristate, 13-acétate (PMA)
Publication date :
16 September 2011
Journal title :
Nutrients
ISSN :
2072-6643
Publisher :
Molecular Diversity Preservation International (MDPI), Basel, Switzerland
Volume :
3
Issue :
9
Pages :
818-838
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
SP Partnership Association Lich Biebergemünder Association for South Development International Help Service IPHD Linz
Griendling, K.K.; Sorescu, D.; Lassègue, B.; Ushio-Fukai, M. Modulation of protein kinase activity and gene expression by reactive oxygen species and their role in vascular physiology and pathophysiology. Arterioscler. Thromb. Vasc. Biol. 2000, 20, 2175-2183.
Valko, M.; Leibfritz, D.; Moncol, J.; Croninc, M.T.D.; Mazur, M.; Telser, J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 2007, 39, 44-84.
Dröge, W. Free radicals in the physiological control of cell function. Physiol. Rev. 2002, 82, 47-95.
Federico, A.; Morgillo, F.; Tuccillo, C.; Ciardiello, F.; Loguercio, C. Chronic inflammation and oxidative stress in human carcinogenesis. Int. J. Cancer 2007, 121, 2381-2386.
Sevanian, A.; Hochstein, P. Mechanisms and consequences of lipid peroxidation in biological systems. Annu. Rev. Nutr. 1985, 5, 365-390.
Bland, J. Biochemical consequences of lipid peroxidation. J. Chem. Educ. 1978, 55, 151-155.
Richter, C. Biophysical consequences of lipid peroxidation in membranes. Chem. Phys. Lipids 1987, 44, 175-189.
Kim, J.A.; Neupane, G.P.; Lee, E.S.; Jeong, B.S.; Park, B.C.; Thapa, B.C. NADPH oxidase inhibitors: A patent review. Expert Opin. Ther. Pat. 2011, 21, 1147-1158.
Murakami, A.; Ohigashi, H. Targeting NOX, INOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals. Int. J. Cancer 2007, 121, 2357-2363.
Heim, K.E.; Tagliaferro, A.R.; Bobilya, D.J. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 2002, 13, 572-584.
Rice-Evans, C.A.; Miller, N.J.; Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 1996, 20, 933-956.
Seifried, H.E.; Anderson, D.E.; Fisher, E.I.; Milner, J.A. A review of the interaction among dietary antioxidants and reactive oxygen species. J. Nutr. Biochem. 2007, 18, 567-579.
Anand, P.; Kunnumakara, A.B.; Sundaram, C.; Harikumar, K.B.; Tharakan, S.T.; Lai, O.S.; Sung, B.; Aggarwal, B.B. Cancer is a preventable disease that requires major lifestyle changes. Pharm. Res. 2008, 25, 2097-2116.
Prior, R.L. Fruits and vegetables in the prevention of cellular oxidative damage. Am. J. Clin. Nutr. 2003, 78, 570S-578S.
Vauzour, D.; Rodriguez-Mateos, A.; Corona, G.; Oruna-Concha, M.J.; Spencer, J.P.E. Polyphenols and human health: Prevention of disease and mechanisms of action. Nutrients 2010, 2, 1106-1131.
Weisburger, J.H. Mechanisms of action of antioxidants as exemplified in vegetables, tomatoes and tea. Food Chem. Toxicol. 1999, 3, 943-948.
Almazan, A.M.; Theberge, R.L. Influence of cassava mosaic virus on cassava leaf-vegetable quality. Trop. Agric. 1989, 66, 305-308.
Kubo, I.; Masuoka, N.; Nihei, K.I.; Burgheim, B. Maniçoba, a quercetin-rich Amazona dish. J. Food Compos. Anal. 2006, 19, 579-588.
Miladiyah, I.; Dayi, F.; Desrini, S. Analgesic activity of ethanolic extract of Manihot esculenta Crantz leaves in mice. Univ. Med. 2011, 30, 3-10.
Wong, S.P.; Leong, L.P.; Koh, J.H.W. Antioxidant activities of aqueous extracts of selected plants. Food Chem. 2006, 99, 775-783.
Suresh, R.; Saravanakumar, M.; Suganyadevi, P. Anthocyanins from Indian cassava (Manihot esculenta Crantz) and its antioxidant properties. Int. J. Pharm. Sci. Res. 2011, 2, 1819-1828.
Katsube, T.; Tabata, H.; Ohta, Y.; Yamasaki, Y.; Anuurad, E.; Shiwaku, K.; Yamana, Y. Screening of antioxidant activity in edible plant products: Comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay. J. Agric. Food Chem. 2004, 52, 2391-2396.
Khomsug, P.; Thongjaroenbuangam, W.; Pakdeenarong, N.; Suttajit, M.; Chantiratikul, P. Antioxidative activities and phenolic content of extracts from Okra (Abelmoschus esculentus L.). Res. J. Biol. Sci. 2010, 5, 310-313.
Adelakun, O.E.; Oyelade, O.J.; Ade-Omowaye, B.I.O.; Adeyemi, I.A.; Van de Venter, M. Chemical composition and the antioxidative properties of Nigerian Okra seed (Abelmoschus esculentus Moench) Flour. Food Chem. Toxicol. 2009, 47, 1123-1126.
Yang, R.-Y.; Tsou, S.C.S.; Lee, T.-C.; Wu, W.-J.; Hanson, P.M.; Kuo, G.; Engle, L.M.; Lai, P.-Y. Distribution of 127 edible plant species for antioxidant activities by two assays. J. Sci. Food Agric. 2006, 86, 2395-2403.
Wright, C.I.; Van-Buren, L.; Kroner, C.I.; Koning, M.M.G. Herbal medicines as diuretics: A review of the scientific evidence. J. Ethnopharmacol. 2007, 114, 1-31.
Imperato, F. Kaempferol-7-O-Rhamnoside-4′-O-glucoside from Pteridium Aquilinum. Phytochemistry 1998, 47, 911-913.
Mendez, J. Dihydrocynnamic acids in Pteridium aquilinum. Food Chem. 2005, 93, 251-252.
Frankel, E.N. Recent advances in lipid oxidation. J. Sci. Food Agric. 1991, 54, 495-511.
Serteyn, D.; Grulke, S.; Franck, T.; Mouithys-Mickalad, A.; Deby-Dupont, G. La myeloperoxidase des neutrophils, une enzyme aux capacities oxydantes. Ann. Méd. Vét. 2003, 147, 79-93.
Beyer, E.M., Jr.; Morgan, P.W. A method for determining the concentration of ethylene in the gas phase of vegetative plant tissues. Plant Physiol. 1970, 46, 352-354.
Deby, C.; Deby-Dupont, G.; Hans, P.; Pincemail, J.; Neuray, J.; Goutier, R. Complementary procedures for pro-and anti-lipoperoxidant activity measurements. Experientia 1983, 39, 1113-1115.
Mouithys-Mickalad, A.; Zheng, S.X.; Deby-Dupont, G.P.; Deby, C.M.T.; Lamy, M.M.; Reginster, J.Y.Y.; Henrontin, Y.E. In vitro study of antioxidant properties of non steroidal anti-inflammatory drugs by chemiluminescence and electron spin resonance (ESR). Free Radic. Res. 2000, 33, 607-621.
Tenant, J.R. Evaluation of the Trypan blue technique for determination of cell viability. Transplantation 1964, 2, 685-694.
Amado, L.L.; Jaramillo, M.D.; Rocha, A.M.; Ferreira, J.L.R.; Garcia, L.M.; Ramos, P.B.; Urrutia, C.; Beltran, C.; Monserrat, J.M. A new method to evaluate total antioxidant capacity against reactive oxygen and nitrogen species (RONS) in aquatic organisms. Comp. Biochem. Physiol. A 2007, 148, S75-S76.
Singleton, V.L.; Orthofer, R.; Lamuela-Raventos, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by average of Folin-Ciocalteu reagent. Methods Enzymol. 1999, 299, 152-178.
Pharmacopée européenne, 4th ed.; DEQM (Direction Européenne de la Qualité du Médicamen) Conseil de l'Europe: Strasbourg, France, 2002; pp. 816-817.
Westby, A. Cassava Utilization, Storage and Small-Scale Processing. In Cassava: Biology, Production and Utilization; Hahn, S.K., Tresh, J.M., Belloti, A.C., Eds.; CABI Publishing: New York, NY, USA, 2001; pp. 281-300.
Montagnac, J.A.; Davis, C.R.; Tanumihardjo, S.A. Processing techniques to reduce toxicity and antinutrients of cassava for use as a staple food. Compr. Rev. Food Sci. Food Saf. 2009, 8, 17-27.
Santos-Buelga, C.; Scalbert, A. Proanthocyanidins and tannin-like compounds-nature, occurrence, dietary intake and effects on nutrition and health. J. Sci. Food Agric. 2000, 80, 1094-1117.
Sharma, M.; Li, L.; Cezlver, J.; Killian, C.; Kovoor, A.; Seeram, N.P. Effects of fruit ellagitannin extracts, ellagic acid, and their colonic metabolite, urolithin a, on wnt signaling. J. Agric. Food Chem. 2010, 58, 3965-3969.
Gutteridge, J.M.C.; Halliwell, B. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem. Sci. 1990, 15, 129-135.
Halliwell, B.; Chirico, S. Lipid peroxidation: its mechanism, measurement and significance. Am. J. Clin. Nutr. 1993, 57, 715S-724S.
Simopoulos, A.P. Essential fatty acids in health and chronic disease. Am. J. Clin. Nutr. 1999, 70, 560S-569S.
Decker, A. Phenolics: Prooxidants or Antioxidants? Nutr. Rev. 1997, 55, 396-398.
Simić, A.; Manojlovic, D.; Šegan, D.; Todorovic, M. Electrochemical behavior and antioxidant and prooxidant activity of natural phenolics. Molecules 2007, 12, 2327-2340.
Ansari, N.M.; Houlihan, L.; Hussain, L.; Pieroni, A. Antioxidant activity of five vegetables traditionally consumed by South-Asian migrants in Bradford, Yorkshire, UK. Phytother. Res. 2005, 19, 907-911.
Oboh, G.; Akindahunshi, A.A. Change in the ascorbic acid, total phenol and antioxidant activity of sun-dried commonly consumed green leafy vegetables in Nigeria. Nutr. Health 2004, 18, 29-36.
Chanock, S.J.; el Benna, J.; Smith, R.M.; Babior, B.M. The respiratory burst oxidase. J. Biol. Chem. 1994, 269, 24519-24522.
Teufelhofer, O.; Weiss, R.M.; Parzefall, W.; Schulte-Hermann, R.; Micksche, M.; Berger, W.; Elbling, L. Promyelytic HL 60 cells express NADPH oxidase and are excellent targets in a rapid spectrophotometric microplate assay for extracellular superoxide. Toxicol. Sci. 2003, 76, 376-383.
Dewanto, V.; Wu, X.; Adom, K.K.; Liu, R.H. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem. 2002, 50, 3010-3014.
Jeong, S.M.; Kim, S.Y.; Kim, D.R.; Jo, S.C.; Nam, K.C.; Ahn, D.U.; Lee, S.C. Effect of heat treatment on the antioxidant activity of extracts of citrus peels. J. Agric. Food Chem. 2004, 52, 3389-3393.
Temitope, A.O.; Olufemi, A.G.; Alaba, F.T. Effect of heat treatment on antioxidant activity of some spices. Cont. J. Food Sci. Technol. 2010, 4, 53-59.
