[en] Blood samples were taken from calves with respiratory disease the first day of examination for determination of the serum concentration of haptoglobin, fibrinogen, alpha-2- and gamma-globulins, and albumin. A clinical examination was performed daily for the duration of the disease. The animals were retrospectively classified in two categories: those animals requiring no treatment or antibiotics alone (group A), and antibiotics associated to anti-inflammatory drugs (group B). The serum proteins were tested in order to check whether they were able to distinguish, on the first day of clinical examination, between calves requiring anti-inflammatory treatment (group B) or not (group A). About 80% of calves were properly classified in both groups by the combined use of the two serum proteins haptoglobin and fibrinogen: these two proteins, and especially haptoglobin, were useful for the identification of calves requiring an anti-inflammatory treatment.
Disciplines :
Biochemistry, biophysics & molecular biology Anatomy (cytology, histology, embryology...) & physiology Veterinary medicine & animal health
Author, co-author :
Humblet, Marie-France ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér.
Coghe, J.
Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Godeau, Jean-Marie ; Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie
Language :
English
Title :
Acute phase proteins assessment for an early selection of treatments in growing calves suffering from bronchopneumonia under field conditions
Publication date :
2004
Journal title :
Research in Veterinary Science
ISSN :
0034-5288
eISSN :
1532-2661
Publisher :
British Veterinary Association, London, United Kingdom
Coghe, J., Uystepruyst, C., Bureau, F., Detilleux, J., Art, T., Lekeux, P., 2000. Validation and prognostic value of plasma lactate measurement in bovine respiratory disease. The Veterinary Journal 160, 139-146.
Conner, J.G., Eckersall, P.D., Doherty, M., Douglas, T.A., 1986. Acute phase response and mastitis in the cow. Research in Veterinary Science 41, 126-128.
Conner, J.G., Eckersall, P.D., Wiseman, A., Aitchison, T.C., Douglas, T.A., 1988. Bovine acute phase response following turpentine injection. Research in Veterinary Science 44, 82-88.
Eckersall, P.D., Conner, J.G., 1988. Bovine and canine acute phase proteins. Veterinary Research Communications 12, 169-178.
Gabay, C., Kushner, I., 1999. Acute-phase proteins and other systemic responses to inflammation. The New England Journal of Medicine 340, 448-454.
Genicot, B., Close, R., Lindsey, J.K., Lekeux, P., 1995. Pulmonary function changes induced by three regimens of bronchodilating agents in calves with acute respiratory distress syndrome. Veterinary Record 137, 183-186.
Godeau, J.-M., Pirlot, A., Rizet, C., Cabanac, S., Schelcher, F., Navetat, H., 2000. Surveys of haptoglobin and fibrinogen changes during a challenge infection with Pasteurella haemolytica in one-week-old calves subjected to an antiobiotherapy. Revue de Médecine Vétérinaire 151, 705.
Godson, D.L., Campos, M., Attah-Poku, S.K., Redmond, M.J., Cordeiro, D.M., Sethi, M.S., Harland, R.J., Babiuk, L.A., 1996. Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease. Veterinary Immunology and Immunopathology 51, 277-292.
Griffin, D., 1997. Economic impact associated with respiratory disease in beef cattle. The Veterinary Clinics of North America: Food Animal Practice 13, 367-378.
Heegaard, P.M.H., Godson, D.L., Toussaint, M.J.M., Tjørnehøj, K., Larsen, L.E., Viuff, B., Rønsholt, L., 2000. The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Veterinary Immunology and Immunopathology 77, 151-159.
Jahoor, F., Sivakumar, B., Delrosario, M., Frazer, E.M., 1996. Isolation of acute-phase proteins from plasma for determination of fractional synthesis rates by a stable isotope tracer technique. Analytical Biochemistry 236, 95-100.
Lockwood, P.W., De Haas, V., Katz, T., 1996. Comparative efficacies of florfenicol and various antibiotics in the treatment of the bovine respiratory disease complex in Europe. In: Symposium on Respiratory Disease at the XIX World Buiatrics Congress, Edinburgh, Scotland, pp. 54-57.
Pierson, R.E., Kainer, R.A., 1980. Clinical classification of pneumonias in cattle. The Bovine Practitioner 15, 73-79.
Salonen, M., Hirvonen, J., Pyorala, S., Sankari, S., Sandholm, M., 1996. Quantitative determination of bovine serum haptoglobin in experimentally induced Escherichia coli mastitis. Research in Veterinary Science 60, 88-91.
Skinner, J.G., Brown, R.A., Roberts, L., 1991. Bovine haptoglobin response in clinically defined field conditions. Veterinary Record 128, 147-149.
Skinner, J.G., Roberts, L., 1994. Haptoglobin as an indicator of infection in sheep. Veterinary Record 134, 33-36.
Thrusfield, M. (Ed.), 1995. Diagnostic Testing. Veterinary Epidemiology, second ed., Blackwell Sciences Ltd., Oxford, England, pp. 266-285.
Van De Weerdt, M-L., Lekeux, P., 1997. Modulation of lung inflammation in the control of bovine respiratory disease. The Bovine Practitioner 31, 19-31.
Wittum, T.E., Young, C.R., Stanker, L.H., Griffin, D.D., Perino, L.J., Littledike, E.T., 1996. Haptoglobin response to clinical respiratory tract disease in feedlot cattle. American Journal of Veterinary Research 57, 646-649.
Young, C.R., Wittum, T.E., Stanker, L.H., Perino, L.J., Griffin, D.D., Littledike, E.T., 1996. Serum haptoglobin concentrations in a population of feedlot cattle. American Journal of Veterinary Research 57, 131-141.
Zweig, M.H., Campbell, G., 1993. Receiver-operating characteristic (R.O.C.) plots: a fundamental evaluation tool in clinical medicine. Clinical Chemistry 39, 561-577.
