Reference : Biocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist e...

	Document type :	Scientific congresses and symposiums : Unpublished conference
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...)
	To cite this reference:	http://hdl.handle.net/2268/98470

Title :	Biocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist effect of Pichia anomala against Botrytis cinerea on apple.
Language :	English
Author, co-author :	Kwasiborski, Anthony [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
	renaut, Jenny [CRPGL luxembourg > > > >]
	Delaplace, Pierre [Université de Liège - ULg > Sciences agronomiques > Biologie végétale >]
	Lepoivre, Philippe [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
	Jijakli, Haissam [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Publication date :	31-Aug-2011
On invitation :	No
Audience :	International
Event name :	9ème International IOBC/WPRS Workshop on pome fruit diseases
Event date :	du 29 aout 2011 au 2 septembre 2011
Event organizer :	Pcfruit, Saint-trond
Event place (city) :	Hasselt
Event country :	Belgique
Abstract :	[en] Pichia anomala strain K was previously identified as an efficient biocontrol agent of the apple pathogen, Botrytis cinerea. Further study demonstrated the complexicity of the mode of action of P. anomala against B. cinerea. A molecular study revealed implication of exo-β-1,3-glucanases in the mode of action of P. anomala but suggested implication of other factors. The present study aims to increase our knowledge of the mode of action of P. anomala strain Kh6 against B. cinerea using an in situ approach allowing interactions between organisms, and the proteomic tool allowing to study the ultime expression of the genome. One wound per apple were covered by a membrane and inoculated by P. anomala then by B. cinerea or not. Samples were collected at different incubation times to identify the responses of P. anomala to the presence of B. cinerea. After extraction, proteins were separated on 2-D gels. Spots influenced were identified by MALDI-ToF. In exponential phase, P. anomala Kh6 orientates its energetic metabolism from the glycolysis to the oxidative phosphorylation and sets up the pentose phosphate pathway. Thanks to this new pathway, P. anomala probably obtains energy and nucleic acids allowing to colonize the wound as fast as in absence of B. cinerea. In stationary phase, no differences in the P. anomala energetic metabolism, in absence and in presence of B. cinerea were observed. During that phase, P. anomala seems to use the alcoholic fermentation in order to face the nutrients impoverishment of the substrate.
Permalink :	http://hdl.handle.net/2268/98470

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