|Reference : Biocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist e...|
|Scientific congresses and symposiums : Poster|
|Life sciences : Phytobiology (plant sciences, forestry, mycology...)|
|Biocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist effect of Pichia anomala against Botrytis cinerea on apple|
|Kwasiborski, Anthony [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]|
|Renaut, Jenny [CRPGL luxembourg > > > >]|
|Lepoivre, Philippe [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]|
|Jijakli, Haissam [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]|
|congrès français de Spectrométrie de Masse et d'Analyse Protéomique|
|du 19 septembre 2011 au 22 septembre 2011|
|Société Française de Spectrométrie de Masse (SFSM) et la Société Française d'Electrophorèse et d'Analyse Protéomique (SFEAP)|
|[en] The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to apply biological control methods. Pichia anomala strain K was previously identified as an efficient biocontrol agent of the main apple pathogens, Botrytis cinerea and Penicillum expansum. Further study demonstrated the complexicity of the mode of action of P. anomala against B. cinerea. A cDNA-AFLP and gene disruption study revealed implication of exo-β-1,3-glucanases in the mode of action of P. anomala strain Kh6 (a haploid form of P. anomala strain K displaying the same biocontrol properties). However, these studies suggested also implication of other factors. The present study aims to increase our knowledge of the mode of action of P. anomala strain Kh6 against B. cinerea using an in situ approach allowing the triple interaction, host/pathogen/antagonist and the proteomic tool allowing to study the ultime expression of the genome without a priori.
One 50mm wound per apple were covered by a membrane and inoculated by a P. anomala suspension then by B. cinerea or not. Samples were collected during the exponential and stationary phase to identify the early and later responses to the presence of B. cinerea. After extraction, proteins were separated on 2-D gels. Spots influenced by the presence of B. cinerea in exponential and stationary phases were identified by MALDI-ToF.
One hundred five and sixty spots of proteins were influenced by the presence of B. cinerea in exponential and stationary phases respectively. In exponential phase, P. anomala Kh6 in absence of B. cinerea uses mainly the glycolysis pathway, whereas in presence of pathogen, it orientates its energetic metabolism to the oxidative phosphorylation and sets up the pentose phosphate pathway. Thanks to this new orientation, P. anomala Kh6 probably obtains energy and nucleic acids allowing to colonize the wound as fast as in absence of B. cinerea and prevents the use of nutrients by the pathogen. In stationary phase, no differences in the P. anomala Kh6 energetic metabolism, in absence and in presence of B. cinerea were observed. During that phase, P. anomala Kh6 seems to use the alcoholic fermentation in order to face the nutrients impoverishment of the substrate.
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