Reference : A multicentric evaluation of IDMS-traceable creatinine enzymatic assays
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/2268/98407
A multicentric evaluation of IDMS-traceable creatinine enzymatic assays
English
Pieroni, Laurence [ > > ]
DELANAYE, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Néphrologie >]
Boutten, Anne [ > > ]
Bargnoux, Anne-Sophie [ > > ]
Rozet, Eric mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Delatour, Vincent [ > > ]
Carlier, Marie-Christine [ > > ]
Hanser, Anne-Marie [ > > ]
CAVALIER, Etienne mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Froissart, Marc [ > > ]
Cristol, Jean-Paul [ > > ]
2011
Clinica Chimica Acta
Elsevier Science
412
2070-2075
Yes (verified by ORBi)
International
0009-8981
Amsterdam
The Netherlands
[en] creatinine ; IDMS traceable ; standardization
[en] Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of those of serum creatinine assays. International recommendations highlight the need for traceable creatinine assays. The French Society of Clinical Biochemistry conducted a study for measuring accuracy of creatinine enzymatic methods. This evaluation involved 25 clinical laboratories. Creatinine was measured in serum pools ranging from 35.9±0.9 μmol/L to 174.5±3.1 μmol/L (IDMS determination) using 12 creatinine enzymatic methods.
For all creatinine values greater than 74.4±1.4 μmol/L, the bias and imprecision did not exceed 5% and 5.9%, respectively. For the lowest value (35.9±0.9 μmol/L), the bias ranged from −1.8 to 9.9% (with one exception). At this level, the imprecision ranged from 1.9 to 7.8%. The true performances of the assays (couples of bias and relative standard deviation), were evaluated using Monte-Carlo simulations. Most of the assays fall within the maximum Total Error of 12% at all concentrations. This study demonstrates substantial improvements in the calibration, traceability and precision of the enzymatic methods, reaching the NKDEP recommendations. Moreover, most of these assays allowed accurate creatinine measurements for creatinine
levels lower than 40 μmol/L.
http://hdl.handle.net/2268/98407

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
a multicentric evaluation of idms.pdfPublisher postprint715.14 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.