ORBi: Soyeurt Hélène - Mid-infrared predictions of lactoferrin content in bovine milk

Reference : Mid-infrared predictions of lactoferrin content in bovine milk


	Document type :	Scientific congresses and symposiums : Paper published in a journal
	Discipline(s) :	Life sciences : Animal production & animal husbandry Life sciences : Genetics & genetic processes Life sciences : Food science
	To cite this reference:	http://hdl.handle.net/2268/98047

Title :	Mid-infrared predictions of lactoferrin content in bovine milk
Language :	English
Author, co-author :	Soyeurt, Hélène [Université de Liège - ULg > Sciences agronomiques > Zootechnie >]
	Bastin, Catherine [Université de Liège - ULg > Sciences agronomiques > Zootechnie >]
	Colinet, Frédéric [Université de Liège - ULg > Sciences agronomiques > Zootechnie >]
	Arnould, Valérie [Université de Liège - ULg > Sciences agronomiques > Zootechnie >]
	Berry, Donagh [Animal and Grassland Research and Innovation Center, TEAGASC, Fermoy, Co. Cork, Ireland > > > >]
	Wall, Eileen [Sustainable Livestock Systems Group, Scottisch Agricultural College, Bush Estate, Penicuik, Midlothian, UK > > > >]
	Gengler, Nicolas [Université de Liège - ULg > Sciences agronomiques > Zootechnie >]
	Dardenne, Pierre [Centre Wallon de Recherches Agronomiques > Département Valorisation des Productions Agricoles > > >]
	McParland, Sinead [Animal and Grassland Research and Innovation Center, TEAGASC, Fermoy, Co. Cork, Ireland > > > >]
Publication date :	2011
Journal title :	Journal of Dairy Science
Publisher :	American Dairy Science Association
Volume :	94
Issue/season :	E-suppl.1
Pages :	714
On invitation :	No
Audience :	International
ISSN :	0022-0302
e-ISSN :	1525-3198
City :	Champaign
Country :	IL
Event name :	ADSA - ASAS Joint Annual Meeting 2011
Event date :	du 10 au 14 juillet 2011
Event organizer :	Federation of Animal Science Societies
Event place (city) :	New Orleans
Event country :	LA
Keywords :	[en] milk ; lactoferrin ; infrared
Abstract :	[en] Lactoferrin (LF) is a glycoprotein present in milk and active in the immune system of cows and humans. Therefore, an inexpensive and rapid analysis to quantify this protein is desirable. A previous study reported the potential to quantify LF from the mid-infrared (MIR) spectrometry from 69 milk samples. Through the European RobustMilk project (www.robustmilk.eu), 3,606 milk samples were collected in Belgium, Ireland, and Scotland from individual cows and analyzed using a MIR MilkoScanFT6000 spectrometer. Milk LF content was quantified using ELISA in duplicate. Average ELISA data with a CV lower than 5% were used. After the detection of spectral and ELISA outliers, the calibration set contained 2,499 samples. An equation to predict LF content from MIR was developed using partial least squared regression. A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. The lactoferrin mean was 159.28 mg/l of milk with a SD of 97.21 mg/l of milk. The calibration (C) coefficient of determination (R2) was equal to 0.73 with a standard error (SE) of calibration of 50.54 mg/l of milk. A cross-validation (CV) was used to assess the robustness of the equation. R2 CV was 0.72 with a SE-CV of 51.16 mg/l of milk. An external validation (V) was conducted on 150 milk samples collected in Belgium. The SE of prediction (SEP) was 59.17 mg/L of milk. The similarity between R2 C and R2CV as well as between SE-C and SE-CV and between SE-CV and SEP confirms the equations developed are robust. The correlation between predicted and measured LF values was 0.71. This lower value compared with the one obtained from the calibration set (0.85) could be explained by the low ELISA reproducibility (16.24% ± 25.51%). If the developed equation is used to clean the validation data set, a total of 16 samples can be deleted. The validation coefficient for these 134 samples increased to 0.82. From these results, the developed equation could be used for screening the dairy cow population for breeding purposes.
Funders :	Commission européenne : Direction générale de l'Agriculture et du Développement rural
Name of the research project :	Robustmilk
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/98047
Other URL :	http://www.jtmtg.org/2011/abstracts/0713.PDF
Mentions required by the publisher for OA :	Publisher copyright and source are acknowledged
Framework Programme / European Project :	FP7 ; 211708 - ROBUSTMILK - Innovative and Practical Breeding Tools for Improved Dairy Products from More Robust Dairy Cattle

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