Reference : Physical and physiological impacts of different foam control strategies during a proc...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/97662
Physical and physiological impacts of different foam control strategies during a process involving hydrophobic substrate for the lipase production by Yarrowia lipolytica
English
Kar, Tambi mailto [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
Destain, Jacqueline mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Delvigne, Frank mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
2012
Bioprocess and Biosystems Engineering
35
4
483-492
Yes (verified by ORBi)
International
1615-7591
1615-7605
[en] The potentialities for the intensification of the process of lipase production by the yeast Yarrowia lipolytica on a renewable hydrophobic substrate (methyloleate) have been investigated. The key factor governing the lipase yield is the intensification of the oxygen transfer rate, considering the fact that Y. lipolytica is a strict aerobe. However, considering the nature of the substrate and the capacity for protein excretion and biosurfactant production of Y. lipolytica, intensification of oxygen transfer rate is accompanied by an excessive formation of foam. Two different foam control strategies have thus been implemented: a classical chemical foam control strategy (CFM) and a mechanical foam control (MFM) based on the Stirring As Foam Disruption (SAFD) principle. The second strategy allows foam control without any modifications of the physico-chemical properties of the broth. However, the MFM system design induced the formation of a persistent foam layer in the bioreactor. This phenomenon has led to the segregation of microbial cells between the foam phase and the liquid phase in the case of the bioreactors operated with MFM control, and induced a reduction at the level of the lipase yield. More interestingly, flow cytometry experiments have shown that residence time of microbial cells in the foam phase tends to induce a dimorphic transition which could potentially explain the reduction of lipase excretion.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/97662

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Final version.pdfPublisher postprint965.9 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.