ORBi: Renoux Virginie - Human papillomavirus entry into NK cells requires CD16 expression and triggers cytotoxic activity and cytokine secretion.

Reference : Human papillomavirus entry into NK cells requires CD16 expression and triggers cytotoxic...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology Human health sciences : Immunology & infectious disease Human health sciences : Oncology
	To cite this reference:	http://hdl.handle.net/2268/97398

Title :	Human papillomavirus entry into NK cells requires CD16 expression and triggers cytotoxic activity and cytokine secretion.
Language :	English
Author, co-author :	Renoux, Virginie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques]
	Bisig, Bettina [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques]
	Langers, Inge [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques]
	Dortu, Estelle [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques]
	Clemenceau, Béatrice [> > > >]
	Thiry, Marc [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
	Deroanne, Christophe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs]
	Colige, Alain [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs]
	Boniver, Jacques [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques]
	Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Jacobs, Nathalie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Publication date :	Nov-2011
Journal title :	European journal of immunology
Volume :	41
Issue/season :	11
Pages :	3240-3252
Audience :	International
ISSN :	0014-2980
e-ISSN :	1521-4141
Keywords :	[en] Natural killer ; Human papillomavirus ; CD16 ; uterine cervical cancer ; Cytotoxic activity ; IFN-gamma ; TNF-alpha ; Squamous cell carcinoma ; Virus-like particles
Abstract :	[en] Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV-infected women clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has evaluated the direct interaction between HPVs and NK cells, a key player in host resistance to viruses and tumors. We demonstrated an NK cell infiltration in HPV-associated pre-neoplastic cervical lesions. Since HPVs cannot grow in vitro, virus-like particles (VLPs) were used as a model for studying the NK cell response against the virus. Interestingly, NK cells displayed higher cytotoxic activity and cytokine production (TNF-alpha and IFN-gamma) in the presence of HPV-VLPs. Using flow cytometry and microscopy we observed that NK cell stimulation was linked to rapid VLP entry into these cells by macropinocytosis. Using CD16(+) and CD16(-) NK cell lines and a CD16-blocking antibody, we demonstrated that CD16 is necessary for HPV-VLP internalization, as well as for degranulation and cytokine production. Thus, we show for the first time that NK cells interact with HPVs and can participate in the immune response against HPV-induced lesions.
Research units :	Giga-Infection, Immunity and Inflammation ; Giga-Cancer
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Télévie
Target :	Researchers ; Students
Permalink :	http://hdl.handle.net/2268/97398
DOI :	10.1002/eji.201141693
Other URL :	http://reflexions.ulg.ac.be/CellulesNK
Commentary :	Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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