Article (Scientific journals)
Development and validation of a ultra-high-performance liquid chromatography-UV method for the detection and quantification of erectile dysfunction drugs and some of their analogues found in counterfeit medicines
Sacré, Pierre-Yves; Deconinck, Eric; Chiap, Patrice et al.
2011In Journal of Chromatography. A, 1218, p. 6439-6447
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Keywords :
Phosphodiesterase type 5 inhibitors; UHPLC; method validation; analogues; counterfeit drugs; accuracy profiles
Abstract :
[en] Pharmaceutical counterfeiting is a permanently growing problem. Control laboratories are constantly analysing counterfeit medicines. In industrialised countries, one of the main counterfeited class of medicines are erectile dysfunction drugs. This paper describes the development and validation of a fast method to detect and quantify the three authorised phosphodiesterase type 5 inhibitors and five analogues. The method is based on the use of a sub-2 microns polar-embedded column with a gradient using acetonitrile as organic modifier and 10 mM ammonium formate buffer (pH 3.5) as aqueous component of the mobile phase. The separation was achieved in less than 4.5 min. The method has also been compared to the registered HPLC method for the assay of Viagra® which was considered as the reference method. The method is also compatible with on-line coupling mass spectrometry and will significantly reduce analysis times and solvent consumption.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Sacré, Pierre-Yves  ;  Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (Bologne)
Deconinck, Eric
Chiap, Patrice ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Mansion, François ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Rozet, Eric ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Courselle, Patricia
De Beer, Jacques
Language :
English
Title :
Development and validation of a ultra-high-performance liquid chromatography-UV method for the detection and quantification of erectile dysfunction drugs and some of their analogues found in counterfeit medicines
Publication date :
2011
Journal title :
Journal of Chromatography. A
ISSN :
0021-9673
eISSN :
1873-3778
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
1218
Pages :
6439-6447
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 August 2011

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