Reference : Development and validation of a ultra-high-performance liquid chromatography-UV method f...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/97169
Development and validation of a ultra-high-performance liquid chromatography-UV method for the detection and quantification of erectile dysfunction drugs and some of their analogues found in counterfeit medicines
English
Sacré, Pierre-Yves mailto [Université de Liège - ULg > > > Doct. sc. bioméd. & pharma. (Bologne)]
Deconinck, Eric [> >]
Chiap, Patrice mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Mansion, François mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Rozet, Eric mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Courselle, Patricia [> >]
De Beer, Jacques [> >]
2011
Journal of Chromatography. A
Elsevier Science
1218
6439-6447
Yes (verified by ORBi)
International
0021-9673
1873-3778
Amsterdam
The Netherlands
[en] Phosphodiesterase type 5 inhibitors ; UHPLC ; method validation ; analogues ; counterfeit drugs ; accuracy profiles
[en] Pharmaceutical counterfeiting is a permanently growing problem. Control laboratories are constantly analysing counterfeit medicines. In industrialised countries, one of the main counterfeited class of medicines are erectile dysfunction drugs. This paper describes the development and validation of a fast method to detect and quantify the three authorised phosphodiesterase type 5 inhibitors and five analogues. The method is based on the use of a sub-2 microns polar-embedded column with a gradient using acetonitrile as organic modifier and 10 mM ammonium formate buffer (pH 3.5) as aqueous component of the mobile phase. The separation was achieved in less than 4.5 min. The method has also been compared to the registered HPLC method for the assay of Viagra® which was considered as the reference method.
The method is also compatible with on-line coupling mass spectrometry and will significantly reduce analysis times and solvent consumption.
WIV-ISP
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/97169
10.1016/j.chroma.2011.07.029

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