Reference : Immunohistochemical investigation of alpha1 (IV) and alpha5 (IV) collagen chains in a br...
Scientific journals : Article
Human health sciences : Dermatology
http://hdl.handle.net/2268/9681
Immunohistochemical investigation of alpha1 (IV) and alpha5 (IV) collagen chains in a broad spectrum of melanocytic tumours.
English
Quatresooz, Pascale mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
Pierard, Gérald mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
2005
Melanoma Research
Lippincott Williams & Wilkins
15
3
161-8
Yes (verified by ORBi)
International
0960-8931
1473-5636
London
United Kingdom
[en] Cell Communication ; Collagen Type IV/analysis ; Humans ; Immunohistochemistry ; Melanoma/chemistry/pathology ; Nevus, Pigmented/chemistry/pathology ; Skin Neoplasms/chemistry/pathology ; Tissue Fixation
[en] BACKGROUND: Cells of melanocytic naevi and cutaneous malignant melanomas (MM) are surrounded by a basement membrane (BM). AIM: To scrutinize any difference between the deposits of alpha1 (IV) and alpha5 (IV) collagen chains in melanocytic naevi and MM. METHODS: A total of 27 common melanocytic naevi, 11 dysplastic naevi, 21 atypical naevi (melanocytomas) including Spitz and non-Spitz types, as well as 24 MM were studied. Their phenotypic and functional characteristics defined by immunohistochemistry using a panel of antibodies, including those directed to the alpha1 (IV), alpha3 (IV) and alpha5 (IV) collagen chains. RESULTS: Almost all naevi and half the melanocytomas exhibited a strong positivity for the alpha1 (IV) collagen chain. By contrast, the remaining melanocytomas and MM presented a heterogeneous staining pattern for the alpha1 (IV) collagen chain. One third of the naevi, 23% of the MM without cutaneous micrometastasis and 83% of MM with cutaneous micrometastasis showed discrete cytoplasmic positivity for the alpha5 (IV) collagen chain. All other melanocytic tumours were negative for this antibody. Rare MM cells in transepidermal migration were stained with the anti-alpha1 (IV) or alpha5 (IV) collagen chain antibodies. No immunoreactivity for the alpha3 (IV) collagen chain was disclosed in any of the samples. CONCLUSION: We report the expression of alpha1 (IV) and alpha5 (IV) collagen chains in naevi and MM. The inconsistent staining pattern for alpha1 (IV) collagen chain in phenotypically atypical melanocytomas and in MM highlight the heterogeneity in both cell differentiation and stroma-tumour interactions. This biological aspect may be related to neoplastic progression and influence metastatic potential.
http://hdl.handle.net/2268/9681

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
IMMUNOHISTOCHEMICAL INVEST*.pdfAuthor postprint46.61 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.