Reference : Expression of alternansucrase in potato plants
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/96482
Expression of alternansucrase in potato plants
English
Jacon, Géraldine mailto [Université de Liège - ULg > > Interface Entreprises-Université >]
Vincken, Jean-Paul [ > > ]
Suurs, Luc [ > > ]
Wang, Denong [ > > ]
Liu, Shaoyi [ > > ]
Visser, Richard [ > > ]
2007
Biotechnology Letters
Kluwer Academic Publishers
29
Yes (verified by ORBi)
International
0141-5492
Dordrecht
The Netherlands
[en] starch ; alternansucrase ; alternan ; transgenic potato ; glucansucrase ; polymer solubility
[en] Alternan, which consists of alternating alpha(1 - 3)/alpha (1 - 6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides NRRL B-1355 in potato. Detection of alternan was performed by enzyme-linked immunosorbent assay in tuber juices, revealing a concentration between 0.3 and 1.2 mg g–1 fresh wt. The Asr transcript levels correlated well with alternan accumulation in tuber juices. It appeared that the expression of sucrose-regulated starch- synthesizing genes (ADP-glucose pyrophosphorylase subunit S and granule-bound starch synthase I) was down-regulated. Despite this, the physico- chemical properties of the transgenic starches were unaltered. These results are compared to those obtained with other transgenic potato plants producing mutan [alpha (1 - 3)-linked glucosyl residues and dextran [alpha (1 - 6)-linked glucosyl residues].
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/96482

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