ORBi: Nelissen Bart - Phylogenetic relationships among filamentous helical cyanobacteria investigated on the basis of 16S ribosomal RNA gene sequence analysis

Reference : Phylogenetic relationships among filamentous helical cyanobacteria investigated on the b...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/95933

Title :	Phylogenetic relationships among filamentous helical cyanobacteria investigated on the basis of 16S ribosomal RNA gene sequence analysis
Language :	English
Author, co-author :	Nelissen, Bart [ > > ]
	Wilmotte, Annick [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]
	Neefs, Jean-Marc [ > > ]
	De Wachter, Rupet [ > > ]
Publication date :	1994
Journal title :	Systematic & Applied Microbiology
Publisher :	Elsevier GmbH, Urban & Fischer Verlag
Volume :	17
Pages :	206-210
Audience :	International
ISSN :	0723-2020
City :	Jena
Country :	Germany
Keywords :	[en] phylogeny ; Arthrospira ; Spirulina ; 16S rRNA
Abstract :	[en] The cyanobacterial genera Spirulina and Arthrospira are both characterized by helical trichomes but their taxonomy is controversial and a phylogenetic study is necessary. The sequence of the 16S rRNA gene and the spacer between the 16S and 23S rRNA genes (ITS) was determined for three filamentous coiled cyanobacteria: Spirulina PCC 6313, Arthrospira PCC 7345 and Arthrospira PCC 8005. A distance tree based on the 16S rRNA sequences was constructed using the neighbor-joining method. This tree shows that the two Arthrospira strains are not closely related to the Spirulina strain but belong to a cluster of strains assigned to the genera Oscillatoria, Lyngbya, and Microcoleus. The strain Spirulina PCC 6313 belongs to a branching containing unicellular cyanobacteria. For the two Arthrospira strains, the sequenced ITS region contains the tRNA(Ile) and tRNA(Ala) genes, whereas the spacer region of strain Spirulina PCC 6313 contains only the tRNA(Ile) gene.
Research units :	Université d'Anvers (UIA)
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Permalink :	http://hdl.handle.net/2268/95933
Other URL :	The cyanobacterial genera Spirulina and Arthrospira are both characterized by helical trichomes but their taxonomy is controversial and a phylogenetic study is necessary. The sequence of the 16S rRNA gene and the spacer between the 16S and 23S rRNA genes (ITS) was determined for three filamentous coiled cyanobacteria: Spirulina PCC 6313, Arthrospira PCC 7345 and Arthrospira PCC 8005. A distance tree based on the 16S rRNA sequences was constructed using the neighbor-joining method. This tree shows that the two Arthrospira strains are not closely related to the Spirulina strain but belong to a cluster of strains assigned to the genera Oscillatoria, Lyngbya, and Microcoleus. The strain Spirulina PCC 6313 belongs to a branching containing unicellular cyanobacteria. For the two Arthrospira strains, the sequenced ITS region contains the tRNA(Ile) and tRNA(Ala) genes, whereas the spacer region of strain Spirulina PCC 6313 contains only the tRNA(Ile) gene.

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