[en] In order to determine the nearly complete
16S rRNA gene sequences of cyanobacteria originating
from nonaxenic cultures, a cyanobacterium-specific
oligonucleotide probe was developed to distinguish polymerase
chain reaction (PCR) products of the cyanobacterial
rRNA operons from those resulting from
amplification of contaminating bacteria. Using this
screening method the 16S rRNA genes of four nonaxenic
filamentous cyanobacterial strains belonging to the genera
Leptolyngbya and OscitIatoria were cloned and sequenced.
For the genus Leptolyngbya, the 16S rRNA
sequence of the axenic strain PCC 73110 was also determined.
Phylogenetic trees were constructed based on
complete and partial sequences. The results show that the
strains Leptolyngbya Jbveolarum Kom~rek 1964/112,
Leptolyngbya sp. VRUC 135 Albertano t985/1, and LeptoIyngbya
boryanum PCC 73110 belong to the same
cluster. Strain Oscillatoria cf. corallinae SAG 8.92,
which contains the rare photosynthetic pigment CUphycoerythrin,
is not closely related to other CUphycoerythrin-
containing cyanobacteria. Oscillatoria
agardhii CYA 18, which is a representative of planktonic Oscillatoria species that form toxic blooms in Norwegian
inland waters, has no close relatives in the tree.
Research center :
Université d'Anvers (UIA)
Disciplines :
Microbiology
Author, co-author :
Nelissen, Bart
De Baere, Raymond
Wilmotte, Annick ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
De Wachter, Rupert
Language :
English
Title :
Phylogenetic Relationships of Nonaxenic Filamentous Cyanobacterial Strains Based on 16S rRNA Sequence Analysis
Alternative titles :
[fr] Relations phylogénétiques de souches cyanobactériennes non axéniques sur base de l'analyse du RNA ribosomique 16S
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