Reference : Analysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Scientific congresses and symposiums : Poster
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/95907
Analysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
English
[fr] Analyse des protéases sécrétées par les racines chez Arabidopsis thaliana et Nicotiana tabacum
Désiron, Carole mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
De Lemos Esteves, Frédéric mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Natalis, Lucie [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale > >]
Périlleux, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
Tocquin, Pierre mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie végétale >]
9-Jun-2011
A0
No
No
International
Plant-Based Vaccines & Antibodies 2011
Du 8juin 2011 au 10 juin 2011
Julian K-C Ma (St George's University of London, UK)
Porto
Portugal
[en] Root secreted protease ; recombinant protein ; plant
[en] Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ('rhizosecretion') greatly simplifies harvest and purification of the product, during whole plant life. However, proteases represent up to 10% of the naturally secreted proteins and are known to significantly decrease the yield of production by rhizosecretion. In this study, we analyzed the rhizosecreted proteases of Arabidopsis thaliana and Nicotiana tabacum. Total rhizosecreted proteins were recovered by salt extraction and the protease activity was assayed in vitro or by zymography. The relative contribution of major protease families to total activity was evaluated with specific inhibitors and revealed significant differences between the two species. The degradation capacity of the root-secreted proteases was further characterized against selected target proteins: BSA and human IgGs.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Région wallone (Grant Waleo3 08/1/6861)
Researchers ; Professionals ; Students ; General public ; Others
http://hdl.handle.net/2268/95907

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