Poster (Scientific congresses and symposiums)
Analysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole; De Lemos Esteves, Frédéric; Natalis, Lucie et al.
2011Plant-Based Vaccines & Antibodies 2011
 

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Keywords :
Root secreted protease; recombinant protein; plant
Abstract :
[en] Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ('rhizosecretion') greatly simplifies harvest and purification of the product, during whole plant life. However, proteases represent up to 10% of the naturally secreted proteins and are known to significantly decrease the yield of production by rhizosecretion. In this study, we analyzed the rhizosecreted proteases of Arabidopsis thaliana and Nicotiana tabacum. Total rhizosecreted proteins were recovered by salt extraction and the protease activity was assayed in vitro or by zymography. The relative contribution of major protease families to total activity was evaluated with specific inhibitors and revealed significant differences between the two species. The degradation capacity of the root-secreted proteases was further characterized against selected target proteins: BSA and human IgGs.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Biochemistry, biophysics & molecular biology
Author, co-author :
Désiron, Carole ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale
De Lemos Esteves, Frédéric  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale
Natalis, Lucie;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale
Périlleux, Claire ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale
Tocquin, Pierre  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale
Language :
English
Title :
Analysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Alternative titles :
[fr] Analyse des protéases sécrétées par les racines chez Arabidopsis thaliana et Nicotiana tabacum
Publication date :
09 June 2011
Number of pages :
A0
Event name :
Plant-Based Vaccines & Antibodies 2011
Event organizer :
Julian K-C Ma (St George's University of London, UK)
Event place :
Porto, Portugal
Event date :
Du 8juin 2011 au 10 juin 2011
Audience :
International
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Région wallone (Grant Waleo3 08/1/6861)
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since 20 July 2011

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