Reference : Nitric oxide-related products and myeloperoxidase in bronchoalveolar lavage fluids fr...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/9573
Nitric oxide-related products and myeloperoxidase in bronchoalveolar lavage fluids from patients with ALI activate NF-kappa B in alveolar cells and monocytes.
English
Nys, Monique mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs - Anesthésie et réanimation >]
Preiser, Jean-Charles mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Deby, Ginette [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
Habraken, Yvette [Université de Liège - ULg > > GIGA-R : Virologie - Immunologie >]
Mathy, Marianne [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Damas, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Lamy, Maurice mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
2005
Vascular Pharmacology
Elsevier Science
43
6
425-33
Yes (verified by ORBi)
International
1537-1891
New York
NY
[en] Biotransformation/drug effects ; Bronchoalveolar Lavage Fluid/chemistry ; Bronchoscopy ; Cell Nucleus/metabolism ; Cells, Cultured ; Cytoplasm/metabolism ; Electrophoretic Mobility Shift Assay ; Humans ; Lactoferrin/metabolism ; Lung Diseases/enzymology/metabolism ; Monocytes/metabolism ; NF-kappa B/metabolism ; Nitrates/metabolism ; Nitric Oxide/metabolism ; Pancreatic Elastase/metabolism ; Peroxidase/metabolism ; Pulmonary Alveoli/cytology/metabolism
[en] An increased production of NO* and peroxynitrite in lungs has been suspected during acute lung injury (ALI) in humans, and recent studies provided evidence for an alveolar production of nitrated compounds. We observed increased concentrations of nitrites/nitrates, nitrated proteins and markers of neutrophil degranulation (myeloperoxidase, elastase and lactoferrine) in the fluids recovered from bronchoalveolar lavage fluids (BALF) of patients with ALI and correlated these changes to the number of neutrophils and the severity of the ALI. We also observed that BALFs stimulated the DNA-binding activity of the nuclear transcription factor kappa B (NF-kappaB) as detected by electrophoretic mobility shift assay in human alveolar cells (A549) and monocytes (THP1). The level of activation of the NF-kappaB-binding activity was correlated to the concentration of nitrated proteins and myeloperoxidase. Furthermore, in vitro studies confirmed that NO*-derived species (peroxynitrite and nitrites) and the neutrophil enzyme myeloperoxidase by themselves increased the activation of NF-kappaB, thereby arguing for an in vivo pathogenetic role of NO*-related products and neutrophil enzymes to human ALI.
http://hdl.handle.net/2268/9573
10.1016/j.vph.2005.08.007

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