Reference : Sex differences in the rapid control of aromatase activity in the quail preoptic area.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Endocrinology, metabolism & nutrition
http://hdl.handle.net/2268/95691
Sex differences in the rapid control of aromatase activity in the quail preoptic area.
English
Konkle, A. T. M. [> > > >]
Balthazart, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
2011
Journal of Neuroendocrinology
Blackwell Publishing
23
5
424-34
Yes (verified by ORBi)
International
0953-8194
1365-2826
Oxford
United Kingdom
[en] Adult male quail show high levels of aromatase activity in the preoptic area-hypothalamus (POA-HYP), which parallels the high number of aromatase-immunoreactive cells and elevated mRNA concentrations detected in this brain region by in situ hybridisation. Interestingly, females display considerably lower aromatase activity than males but have almost equal numbers of aromatase-immunoreactive cells and express similar levels of aromatase mRNA. Aromatase activity in the male POA-HYP can be rapidly regulated by calcium-dependent phosphorylations, in the absence of changes in enzyme concentration. In the present study, we investigated whether aromatase activity is differentially regulated by phosphorylations in males and females. A linear increase in accumulation of aromatisation products was observed in both sexes as a function of time but the rate of conversion was slower in females. Saturation analysis confirmed the lower maximum velocities (V(max) ) in females but indicated a similar affinity (K(m) ) in both sexes. Aromatase activity in females reacted differentially to manipulations of intracellular calcium. In particular, chelating calcium with ethylene glycol tetraacetic acid (EGTA) resulted in a larger increase of enzymatic activity in males than in females, especially in the presence of ATP. A differential reaction to kinase inhibitors was also observed between males and females (i.e. a larger increase in aromatase activity in females than in males after exposure to specific inhibitors). These findings suggest that the nature of aromatase is conserved between the sexes, although the control of its activity by calcium appears to be different. Additional characterizations of intracellular calcium in both sexes would therefore be appropriate to better understand aromatase regulation.
http://hdl.handle.net/2268/95691
10.1111/j.1365-2826.2011.02121.x
(c) 2011 The Authors. Journal of Neuroendocrinology (c) 2011 Blackwell Publishing Ltd.

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