Reference : Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/95603
Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters
English
Ledent, P. [> > > >]
Duez, Colette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines]
Vanhove, M. [> > > >]
Lejeune, Annabelle mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Fonze, E. [> > > >]
Charlier, Paulette mailto [Université de Liège - ULg > Département des sciences de la vie > Cristallographie des macromolécules biologiques >]
RhaziFilali, F. [> > > >]
Thamm, Iris mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Guillaume, G. [ > > ]
Samyn, B. [> > > >]
Devreese, B. [> > > >]
VanBeeumen, J. [> > > >]
LamotteBrasseur, J. [Université de Liège - ULg > > Centre d'ingénierie des protéines]
Frère, Jean-Marie mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
1997
FEBS Letters
Elsevier Science
413
2
194-196
International
0014-5793
Amsterdam
The Netherlands
[en] The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed. (C) 1997 Federation of European Biochemical Societies.
http://hdl.handle.net/2268/95603

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