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Insights into the metagenomic approach : identification and characterization of cellulases involved in bacterial cellulose synthesis
Berlemont, Renaud; Delsaute, Maud; Galleni, Moreno
2010239th American Chemical Society National Meeting & Exposition
 

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Keywords :
cellulose; metagenomic
Abstract :
[en] the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 1C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Berlemont, Renaud ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Delsaute, Maud ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Language :
English
Title :
Insights into the metagenomic approach : identification and characterization of cellulases involved in bacterial cellulose synthesis
Publication date :
22 March 2010
Event name :
239th American Chemical Society National Meeting & Exposition
Event organizer :
American Chemical Society
Event place :
San Francisco, United States - California
Event date :
21-25/3/2010
By request :
Yes
Audience :
International
Name of the research project :
GeneHunt
Funders :
ACS - American Chemical Society [US-DC]
Available on ORBi :
since 11 July 2011

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