Reference : Insights into the metagenomic approach : identification and characterization of cellulas...
Scientific congresses and symposiums : Unpublished conference
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/95402
Insights into the metagenomic approach : identification and characterization of cellulases involved in bacterial cellulose synthesis
English
Berlemont, Renaud [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
Delsaute, Maud [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
Galleni, Moreno mailto [Université de Liège - ULg > Département des sciences de la vie > Macromolécules biologiques >]
22-Mar-2010
No
Yes
International
239th American Chemical Society National Meeting & Exposition
21-25/3/2010
American Chemical Society
San Francisco
USA, Ca
[en] cellulose ; metagenomic
[en] the mining of an Antarctic soil sample by functional metagenomics allowed the
isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new
enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri
(Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and
purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and
cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal
dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained
significantly active when temperature decreased (18% of activity at 10 1C). It is interesting that
RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover,
by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1
protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as
the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and
Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.
Centre d'Ingénierie des Protéines - CIP
ACS
GeneHunt
Researchers ; Professionals
http://hdl.handle.net/2268/95402

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