ORBi: Bafort Françoise - Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydase system against phytopathogens

Reference : Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydas...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Life sciences : Agriculture & agronomy
	To cite this reference:	http://hdl.handle.net/2268/95376

Title :	Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydase system against phytopathogens
Language :	English
Author, co-author :	Bafort, Françoise [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
	Lepoivre, Philippe [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
	Jijakli, Haissam [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Other contributor :	Perraudin, Jean-Paul [Taradon Laboratory > > > >]
Publication date :	24-May-2011
Page number :	A0
On invitation :	No
Audience :	International
Event name :	63rd International Symposium on Crop Protection
Event date :	24 may 2011
Event organizer :	Ghent university
Event place (city) :	Ghent
Event country :	Belgium
Keywords :	[en] Lactoperoxydase ; phytopathogens ; Elisa screening
Abstract :	[en] The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of hydrogen peroxide in hypoiodite (OI-) and hypothiocyanite (OSCN-) ions. Both ions have inhibitory action against pathogenic bacteria, fungi and viruses. Until now, the ions OI- and OSCN- applied separately from the LPS were not tested against plant pathogens. Seventeen KI/KSCN/H2O2 ratios with 3 dilutions for each one were tested against Botrytiscinerea. Results showed that the ions OI- and OSCN- gives a better % of inhibition with: -high concentration of KI+KSCN, -a ratio of KI/KSCN of 4.5 and -a ratioof (KI+KSCN)/H2O2 near 1. Increased dilution combined with lower KI+KSCN concentration reduced the efficacy of the ions. From the screening against B.cinerea, 4 ratios of KI+KSCN were selected and tested against 6 other phytopathogens. Results showed that the OI- and OSCN- ions give 87 and 100% inhibition against Penicillium expansum and Verticillium dahlia respectively. The inhibitory activity of the ions solution was ranged between 70 and 79% regarding Fusarium avenaceum, Phytophthora infestans and Colletotrichum lindemuthanium. The ions OI- and OSCN- showed an insufficient inhibitory activity against Septoria tritici. The screening method highlighted that high KI/KSCN ratios and low dilution were more efficient against phytopathogens. The efficient ratios will be further evaluated in vivo.
Funders :	Taradon Laboratory
Name of the research project :	Nouvelle Application du système Lactoperoxydase pour la protection des plantes
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/95376

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