|Reference : Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydas...|
|Scientific congresses and symposiums : Poster|
|Life sciences : Agriculture & agronomy|
|Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydase system against phytopathogens|
|Bafort, Françoise [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]|
|Lepoivre, Philippe [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]|
|Jijakli, Haissam [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]|
|Perraudin, Jean-Paul [Taradon Laboratory > > > >]|
|63rd International Symposium on Crop Protection|
|24 may 2011|
|[en] Lactoperoxydase ; phytopathogens ; Elisa screening|
|[en] The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of hydrogen peroxide in hypoiodite (OI-) and hypothiocyanite (OSCN-) ions. Both ions have inhibitory action against pathogenic bacteria, fungi and viruses. Until now, the ions OI- and OSCN- applied separately from the LPS were not tested against plant pathogens. Seventeen KI/KSCN/H2O2 ratios with 3 dilutions for each one were tested against Botrytiscinerea. Results showed that the ions OI- and OSCN- gives a better % of inhibition with:
-high concentration of KI+KSCN,
-a ratio of KI/KSCN of 4.5 and
-a ratioof (KI+KSCN)/H2O2 near 1.
Increased dilution combined with lower KI+KSCN concentration reduced the efficacy of the ions. From the screening against B.cinerea, 4 ratios of KI+KSCN were selected and tested against 6 other phytopathogens. Results showed that the OI- and OSCN- ions give 87 and 100% inhibition against Penicillium expansum and Verticillium dahlia respectively. The inhibitory activity of the ions solution was ranged between 70 and 79% regarding Fusarium avenaceum, Phytophthora infestans and Colletotrichum lindemuthanium. The ions OI- and OSCN- showed an insufficient inhibitory activity against Septoria tritici. The screening method highlighted that high KI/KSCN ratios and low dilution were more efficient against phytopathogens. The efficient ratios will be further evaluated in vivo.
|Nouvelle Application du système Lactoperoxydase pour la protection des plantes|
|Researchers ; Professionals ; Students|
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