Reference : Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxy...
Scientific congresses and symposiums : Poster
Life sciences : Agriculture & agronomy
Screening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydase system against phytopathogens
Bafort, Françoise mailto [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Lepoivre, Philippe mailto [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Jijakli, Haissam mailto [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Perraudin, Jean-Paul mailto [Taradon Laboratory > > > >]
63rd International Symposium on Crop Protection
24 may 2011
Ghent university
[en] Lactoperoxydase ; phytopathogens ; Elisa screening
[en] The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of hydrogen peroxide in hypoiodite (OI-) and hypothiocyanite (OSCN-) ions. Both ions have inhibitory action against pathogenic bacteria, fungi and viruses. Until now, the ions OI- and OSCN- applied separately from the LPS were not tested against plant pathogens. Seventeen KI/KSCN/H2O2 ratios with 3 dilutions for each one were tested against Botrytiscinerea. Results showed that the ions OI- and OSCN- gives a better % of inhibition with:
-high concentration of KI+KSCN,
-a ratio of KI/KSCN of 4.5 and
-a ratioof (KI+KSCN)/H2O2 near 1.
Increased dilution combined with lower KI+KSCN concentration reduced the efficacy of the ions. From the screening against B.cinerea, 4 ratios of KI+KSCN were selected and tested against 6 other phytopathogens. Results showed that the OI- and OSCN- ions give 87 and 100% inhibition against Penicillium expansum and Verticillium dahlia respectively. The inhibitory activity of the ions solution was ranged between 70 and 79% regarding Fusarium avenaceum, Phytophthora infestans and Colletotrichum lindemuthanium. The ions OI- and OSCN- showed an insufficient inhibitory activity against Septoria tritici. The screening method highlighted that high KI/KSCN ratios and low dilution were more efficient against phytopathogens. The efficient ratios will be further evaluated in vivo.
Taradon Laboratory
Nouvelle Application du système Lactoperoxydase pour la protection des plantes
Researchers ; Professionals ; Students

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