Reference : Perturbation of actin dynamics induces NF-kappa B activation in myelomonocytic cells ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/9518
Perturbation of actin dynamics induces NF-kappa B activation in myelomonocytic cells through an NADPH oxidase-dependent pathway
English
Kustermans, Gaëlle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
El Benna, Jamel [INSERM, Paris, france > > > >]
Piette, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > Virologie - Immunologie >]
Legrand, Sylvie mailto [Université de Liège - ULg > > GIGA-R : Virologie - Immunologie >]
15-Apr-2005
Biochemical Journal
Portland Press
387
Pt 2
531-540
Yes (verified by ORBi)
International
0264-6021
1470-8728
London
United Kingdom
[en] actin cytoskeleton ; myelomonocytic cells ; nuclear factor kappa B (NF-kappa B) ; reactive oxygen species (ROS)
[en] Although several reports showed the effect of compounds disrupting microtubules on NF-kappa B (nuclear factor kappa B) activation, nothing is known about agents perturbing actin dynamics. In the present study, we have shown that actin cytoskeleton disruption induced by actin-depolymerizing agents such as cytochalasin D and latrunculin B and actin-polymerizing compounds such as jasplakinolide induced NF-kappa B activation in myelomonocytic cells. The transduction pathway involved the I kappa B (inhibitory kappa B) kinase complex and a degradation of I kappa B alpha. We have shown that NF-kappa B activation in response to the perturbation of actin dynamics required reactive oxygen species. as demonstrated by the effect of antioxidants. Actin cytoskeleton disruption by cytochalasin D induced O-2(-) release from human monocytes, through the activation of the NADPH oxidase, as confirmed by the phosphorylation and by the membrane translocation of p47(phox). NF-kappa B activation after actin cytoskeleton disruption could be physiologically relevant during monocyte activation and/or recruitment into injured tissues, where cellular attachment, migration and phagocytosis result in cyclic shifts in cytoskeletal organization and disorganization.
Giga-Signal Transduction
IAP 5/12
Modulation of the NF-KappaB activation pathways by the actin cytoskeleton
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/9518
also: http://hdl.handle.net/2268/100335
10.1042/BJ20041318

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