ORBi: Kustermans Gaëlle - Actin cytoskeleton differentially modulates NF-κB-mediated IL-8 expression in myelomonocytic cells

Reference : Actin cytoskeleton differentially modulates NF-κB-mediated IL-8 expression in myelomonoc...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/9514

Title :	Actin cytoskeleton differentially modulates NF-κB-mediated IL-8 expression in myelomonocytic cells
Language :	English
Author, co-author :	Kustermans, Gaëlle [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	El Mjiyad, Nadia [Université de Liège - ULg > Département des sciences de la vie > Virologie - Immunologie >]
	Horion, Julie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Jacobs, Nathalie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Piette, Jacques [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - GIGA-Research >]
	Legrand-Poels, Sylvie [Université de Liège - ULg > > GIGA-R : Virologie - Immunologie >]
Publication date :	2008
Journal title :	Biochemical Pharmacology
Publisher :	Elsevier Science
Volume :	76(10)
Pages :	1214-1228
Audience :	International
ISSN :	0006-2952
City :	Oxford
Country :	United Kingdom
Keywords :	[en] actin cytoskeleton ; interleukin-8 ; NF-kappa B
Abstract :	[en] Many physiopathological events such as phagocytosis, pathogen invasion, cellular adhesion and chemotaxis governed by actin-based cytoskeleton are often accompanied by nuclear factor kB (NF-kB) activation and expression of pro-inflammatory genes. In the present study, we demonstrated that reorganization of actin cytoskeleton induced by Cytochalasin D (CytD), an actin-polymerization inhibitor, enhanced il-8 gene expression induced by TNFa and LPS in HL-60 monocyte-like cells. Both transcriptional and post-transcriptional mechanisms were involved. CytD potentiated NF-kB-mediated transcription induced by both TNFa and LPS but via different mechanisms. In the case of LPS, the perturbation of actin dynamics increased the TLR4 levels at the cell membrane and consequently enhanced the IKK complex activation and NF-kB nuclear translocation. However, the canonical pathway involving the IKK complex and leading to the NF-kB translocation into the nucleus was not affected by actin remodelling in the case of TNFa. Interestingly, actin disruption primed p65 phosphorylation induced by TNFa and LPS, on Ser276 and Ser536, respectively, which suggested actin cytoskeleton could also modulate p65 transactivating activity.
Research units :	Giga-Signal Transduction
Funders :	PAI 5/12 , FNRS
Name of the research project :	Modulation of the NF-KappaB activation pathways by the actin cytoskeleton
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/9514

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