Article (Scientific journals)
Actin cytoskeleton differentially modulates NF-κB-mediated IL-8 expression in myelomonocytic cells
Kustermans, Gaëlle; El Mjiyad, Nadia; Horion, Julie et al.
2008In Biochemical Pharmacology, 76(10), p. 1214-1228
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Keywords :
actin cytoskeleton; interleukin-8; NF-kappa B
Abstract :
[en] Many physiopathological events such as phagocytosis, pathogen invasion, cellular adhesion and chemotaxis governed by actin-based cytoskeleton are often accompanied by nuclear factor kB (NF-kB) activation and expression of pro-inflammatory genes. In the present study, we demonstrated that reorganization of actin cytoskeleton induced by Cytochalasin D (CytD), an actin-polymerization inhibitor, enhanced il-8 gene expression induced by TNFa and LPS in HL-60 monocyte-like cells. Both transcriptional and post-transcriptional mechanisms were involved. CytD potentiated NF-kB-mediated transcription induced by both TNFa and LPS but via different mechanisms. In the case of LPS, the perturbation of actin dynamics increased the TLR4 levels at the cell membrane and consequently enhanced the IKK complex activation and NF-kB nuclear translocation. However, the canonical pathway involving the IKK complex and leading to the NF-kB translocation into the nucleus was not affected by actin remodelling in the case of TNFa. Interestingly, actin disruption primed p65 phosphorylation induced by TNFa and LPS, on Ser276 and Ser536, respectively, which suggested actin cytoskeleton could also modulate p65 transactivating activity.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kustermans, Gaëlle ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
El Mjiyad, Nadia ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Horion, Julie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Jacobs, Nathalie  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - GIGA-Research
Legrand-Poels, Sylvie ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
Language :
English
Title :
Actin cytoskeleton differentially modulates NF-κB-mediated IL-8 expression in myelomonocytic cells
Publication date :
2008
Journal title :
Biochemical Pharmacology
ISSN :
0006-2952
eISSN :
1873-2968
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
76(10)
Pages :
1214-1228
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Modulation of the NF-KappaB activation pathways by the actin cytoskeleton
Funders :
PAI 5/12 , FNRS
Available on ORBi :
since 24 March 2009

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