ORBi: Borbone Nicola - d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:C):G(:C):G(:C) octads

Reference : d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:C):...


	Document type :	Scientific journals : Article
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Chemistry Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/95075

Title :	d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:C):G(:C):G(:C) octads
Language :	English
Author, co-author :	Borbone, Nicola [University of Naples Federico II, 80131 Naples, Italy > > > >]
	Amato, Jussara [University of Naples Federico II, 80131 Naples, Italy > > > >]
	Oliviero, Giorgia [University of Naples Federico II, 80131 Naples, Italy > > > >]
	D'Atri, Valentina [University of Naples Federico II, 80131 Naples, Italy > > > >]
	Gabelica, Valérie [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	De Pauw, Edwin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Piccialli, Gennaro [University of Naples Federico II, 80131 Naples, Italy > > > >]
	Mayol, Luciano [University of Naples Federico II, 80131 Naples, Italy > > > >]
Publication date :	2011
Journal title :	Nucleic Acids Research
Publisher :	Oxford University Press
Audience :	International
ISSN :	0305-1048
e-ISSN :	1362-4962
City :	Oxford
Country :	United Kingdom
Keywords :	[en] mass spectrometry ; nucleic acids ; non-covalent ; G-quadruplex ; electrospray ; FTICR
Abstract :	[en] Among non-canonical DNA secondary structures, G-quadruplexes are currently widely studied because of their probable involvement in many pivotal biological roles, and for their potential use in nanotechnology. The overall quadruplex scaffold can exhibit several morphologies through intramolecular or intermolecular organization of G-rich oligodeoxyribonucleic acid strands. In particular, several G-rich strands can form higher order assemblies by multimerization between several G-quadruplex units. Here, we report on the identification of a novel dimerization pathway. Our Nuclear magnetic resonance, circular dichroism, UV, gel electrophoresis and mass spectrometry studies on the DNA sequence dCGGTGGT demonstrate that this sequence forms an octamer when annealed in presence of K+ or NH4+ ions, through the 5′-5′ stacking of two tetramolecular G-quadruplex subunits via unusual G(:C):G(:C):G(:C):G(:C) octads.
Research units :	Giga-Systems Biology and Chemical Biology
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/95075
DOI :	10.1093/nar/gkr489
Mentions required by the publisher for OA :	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Funding for open access charge: Italian M.U.R.S.T. (P.R.I.N. 2007).

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