Reference : d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/95075
d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:C):G(:C):G(:C) octads
English
Borbone, Nicola [University of Naples Federico II, 80131 Naples, Italy > > > >]
Amato, Jussara [University of Naples Federico II, 80131 Naples, Italy > > > >]
Oliviero, Giorgia [University of Naples Federico II, 80131 Naples, Italy > > > >]
D'Atri, Valentina [University of Naples Federico II, 80131 Naples, Italy > > > >]
Gabelica, Valérie mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Piccialli, Gennaro [University of Naples Federico II, 80131 Naples, Italy > > > >]
Mayol, Luciano [University of Naples Federico II, 80131 Naples, Italy > > > >]
2011
Nucleic Acids Research
Oxford University Press
Yes (verified by ORBi)
International
0305-1048
1362-4962
Oxford
United Kingdom
[en] mass spectrometry ; nucleic acids ; non-covalent ; G-quadruplex ; electrospray ; FTICR
[en] Among non-canonical DNA secondary structures, G-quadruplexes are currently widely studied because of their probable involvement in many pivotal biological roles, and for their potential use in nanotechnology. The overall quadruplex scaffold can exhibit several morphologies through intramolecular or intermolecular organization of G-rich oligodeoxyribonucleic acid strands. In particular, several G-rich strands can form higher order assemblies by multimerization between several G-quadruplex units. Here, we report on the identification of a novel dimerization pathway. Our Nuclear magnetic resonance, circular dichroism, UV, gel electrophoresis and mass spectrometry studies on the DNA sequence dCGGTGGT demonstrate that this sequence forms an octamer when annealed in presence of K+ or NH4+ ions, through the 5′-5′ stacking of two tetramolecular G-quadruplex subunits via unusual G(:C):G(:C):G(:C):G(:C) octads.
Giga-Systems Biology and Chemical Biology
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers
http://hdl.handle.net/2268/95075
10.1093/nar/gkr489
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/
by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Funding for open
access charge: Italian M.U.R.S.T. (P.R.I.N. 2007).

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