Article (Scientific journals)
Bioconversion of a L-Carnitin Precursor in a One- or Two-Phase System
Bare, Ghislain; Jacques, Philippe; Hubert, Jean-Benoit et al.
1991In Applied Biochemistry and Biotechnology, 28-29 (Spring), p. 445-56
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Keywords :
L-carnitin; bioconversion; Saccharomyces cerevisiae
Abstract :
[en] The ability of the yeast Saccharomyces cerevisiae to bioconvert stereo-selectively octyl-4-chloroacetoacetate (OCA) into the corresponding chiral alcohol, precursor of L-carnitin, an important physiological agent, was investigated. In a monophasic system with free cells, more than 90% of OCA (0.018 M) bioconversion have been reached after 6 h (enantiomeric excess for the R form, eeR:97%). Immobilized cells in alginate beads were less efficient in conversion of OCA than free cells. In a two-phase system with free cells, the level of reduction of OCA (0.018 M) reached 85% after 48 h. With a medium containing a higher OCA concentration (0.270 M), 41% of this product were bioconverted after the same period. On the other hand, immobilized cells did not show any significant bioconversion of OCA in two-phase reactors. The limiting factor of these reactors in the regeneration of the cofactors involved in the OCA reduction.
Disciplines :
Microbiology
Author, co-author :
Bare, Ghislain
Jacques, Philippe ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Hubert, Jean-Benoit
Rikir, Rafaèle
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Bioconversion of a L-Carnitin Precursor in a One- or Two-Phase System
Publication date :
1991
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press, United States - New Jersey
Volume :
28-29
Issue :
Spring
Pages :
445-56
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 January 2010

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