Article (Scientific journals)
Integrated on-line sample clean-up using cation exchange restricted access sorbent for the LC determination of atropine in human plasma coupled to UV detection
Rbeida, O.; Christiaens, B.; Hubert, Philippe et al.
2005In Journal of Pharmaceutical and Biomedical Analysis, 36 (5), p. 947-954
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Keywords :
atropine; plasma; sample preparation; cation exchange restricted access material reversed-phase liquid chromatography; column-switching
Abstract :
[en] A new, simple and fully automated liquid chromatographic (LC) method with UV detection has been developed for the direct determination of atropine in plasma. Sample clean-up was based on the use of cation exchange restricted access material (RAM) in a pre-column, coupled to LC by means of a column switching system. After direct injection of a 200 microl-volume of plasma sample, the biological matrix was washed out for 10 min using a washing liquid composed of 2 mM lithium perchlorate adjusted to pH 3.0 and methanol (97:3; v/v). By rotation of the switching valve, atropine was then eluted in the back-flush mode for 2 min and transferred to the analytical column packed with octadecyl silica by the LC mobile phase constituted of a mixture of acetonitrile and potassium phosphate buffer (pH 3.0; 50 mM) containing 2 mM sodium heptanesulfonate (16:84; v/v). The UV detection was performed at 220 nm. The method was validated according to a new approach based on accuracy profile over a concentration range from 25 ng/ml, corresponding to the limit of quantitation, to 1000 ng/ml. The method was then applied for the determination of atropine in plasma after intravenous administration to hospitalised patients.
Disciplines :
Pharmacy, pharmacology & toxicology
Chemistry
Author, co-author :
Rbeida, O.
Christiaens, B.
Hubert, Philippe  ;  Université de Liège - ULiège > Chimie analytique
Lubda, D.
Boos, K. S.
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Chiap, Patrice ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Integrated on-line sample clean-up using cation exchange restricted access sorbent for the LC determination of atropine in human plasma coupled to UV detection
Publication date :
04 January 2005
Journal title :
Journal of Pharmaceutical and Biomedical Analysis
ISSN :
0731-7085
eISSN :
1873-264X
Publisher :
Pergamon-Elsevier Science Ltd, Oxford, United Kingdom
Volume :
36
Issue :
5
Pages :
947-954
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 March 2009

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