Article (Scientific journals)
The spontaneous ability of normal human IgG to inhibit the Fc receptors of normal human monocytes is related to their binding capacity to lectins
Malaise, Michel; Franchimont, P.; Gomez, Florie et al.
1987In Clinical Immunology and Immunopathology, 45 (1), p. 1-16
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Keywords :
Immunoglobulin G; Lectins; Peanut Agglutinin; Receptors, Fc; Concanavalin A
Abstract :
[en] The lectin-binding capacity of 96 normal human IgG, assessed by solid-phase radioimmunoassay, strikingly varied according to the lectin considered. Indeed, half of the IgG samples exhibited peanut agglutinin (PNA)- and pokeweed mitogen-specific binding capacities superior or equal to 4%, whereas less than 15% of IgG similarly bound to concanavalin A (Con A) and to phytohemagglutinin. The ability of those IgG to inhibit the Fc receptor (Fc-R) function of human monocytes, measured by a classical rosette assay, was inversely correlated to their binding ratios to PNA and Con A only. By affinity chromatography, three groups of IgG were separated: the IgG purified on agarose-PNA columns slightly reduced the Fc-R function (40-45% inhibition); the IgG purified on Sepharose-Con A columns exhibited the highest inhibitory properties (80-85% inhibition); the IgG that did not bind to PNA and Con A columns possessed intermediate inhibitory properties (65-70% inhibition). The different effect of IgG on Fc receptors was conserved when monocytes were first treated by trypsin and was unrelated to their specific binding to human monocytes, to their subclasses, and to their C1q- or protein A-binding capacities. Incubation of monocytes with D-galactose (10 mM) significantly improved their capacity to form IgG rosettes, whereas their incubation with D-mannose (10 mM) significantly reduced the Fc-R function. Scatchard plots of 125I-IgG1 myeloma protein binding to monocytes were linear under basal conditions, as well as after a prior incubation of the cells with D-galactose or D-mannose. Monocytes bound about 16,000 molecules of IgG1 per cell in each instance. In contrast, the mean association constant (Ka) for IgG1 binding was 2.59 +/- 0.50 X 10(8) M-1 under basal conditions, 4.4 +/- 0.75 X 10(8) M-1 after D-galactose incubation, and 1.35 +/- 0.50 X 10(8) M-1 after D-mannose incubation. These data suggest that the level of human monocyte Fc-R function blockade induced by human IgG depends mainly on the presence of "accessible" galactosyl or mannosyl residues in the Fc domain and that the modulation of the Fc-R function induced by these carbohydrates is due to a change in the affinity rather than in the number of single class of high-affinity binding sites.
Disciplines :
Rheumatology
Author, co-author :
Malaise, Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
Franchimont, P.
Gomez, Florie ;  Université de Liège - ULiège > Chirurgie abdominale- endocrinienne et de transplantation
Bouillenne, C.
Mahieu, P. R.
Language :
English
Title :
The spontaneous ability of normal human IgG to inhibit the Fc receptors of normal human monocytes is related to their binding capacity to lectins
Publication date :
1987
Journal title :
Clinical Immunology and Immunopathology
ISSN :
0090-1229
Publisher :
Academic Press, Orlando, United States - Florida
Volume :
45
Issue :
1
Pages :
1-16
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 June 2011

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