ORBi: Pirottin Dimitri - Transgenic engineering of male-specific muscular hypertrophy.

Reference : Transgenic engineering of male-specific muscular hypertrophy.


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Genetics & genetic processes
	To cite this reference:	http://hdl.handle.net/2268/94431

Title :	Transgenic engineering of male-specific muscular hypertrophy.
Language :	English
Author, co-author :	Pirottin, Dimitri [Université de Liège - ULg > Département de productions animales > Génétique factorielle et moléculaire > >]
	Grobet, Luc [Université de Liège - ULg > Département de morphologie et pathologie > Embryologie >]
	Adamantidis, Antoine [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	Farnir, Frédéric [Université de Liège - ULg > Département de productions animales > Biostatistique, économie, sélection animale >]
	Herens, Christian [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
	Daa Schroder, Henrik [Odense Universiteitshospital - OUH > Institute of Pathology > > >]
	Georges, Michel [Université de Liège - ULg > Département de productions animales > Génétique factorielle et moléculaire > >]
Publication date :	2005
Journal title :	Proceedings of the National Academy of Sciences of the United States of America
Publisher :	National Academy of Sciences
Volume :	102
Issue/season :	18
Pages :	6413-8
Audience :	International
ISSN :	0027-8424
e-ISSN :	1091-6490
City :	Washington
Country :	DC
Keywords :	[en] Animals ; Body Weights and Measures ; Breeding/methods ; DNA-Binding Proteins/genetics ; Gene Targeting/methods ; Genetic Engineering/methods ; Genetic Vectors/genetics ; Male ; Mice ; Mice, Transgenic ; Muscle Development/genetics ; Muscle, Skeletal/growth & development ; Myosin Light Chains/genetics ; Myostatin ; Nuclear Proteins/genetics ; Promoter Regions, Genetic/genetics ; Sex-Determining Region Y Protein ; Totipotent Stem Cells/metabolism ; Transcription Factors/genetics ; Transforming Growth Factor beta/genetics/metabolism ; Y Chromosome/genetics
Abstract :	[en] Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.
Permalink :	http://hdl.handle.net/2268/94431
DOI :	10.1073/pnas.0502426102

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