ORBi: Kohnen Stephan - Catalytic activation of copper(II) salts on the reaction of peroxynitrite with propofol in alkaline medium

Reference : Catalytic activation of copper(II) salts on the reaction of peroxynitrite with propofol ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/940

Title :	Catalytic activation of copper(II) salts on the reaction of peroxynitrite with propofol in alkaline medium
Language :	English
Author, co-author :	Kohnen, Stephan [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Halusiak, Emilie [Université de Liège - ULg > > Centre d'études et de rech. sur les macromolécules (CERM) >]
	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Deby-Dupont, G. [> > > >]
	Deby, Christiane [Centre Hospitalier Universitaire de Liège - CHU > > Administration des patients - Admission des hospitalisés >]
	Hans, Pol [Université de Liège - ULg > Département des sciences cliniques > Anesthésie et réanimation]
	Lamy, Maurice [Université de Liège - ULg > Département des sciences cliniques > Anesthésie et réanimation]
	Noels, A. F. [> > > >]
Publication date :	Jun-2005
Journal title :	Nitric Oxide
Publisher :	Academic Press Inc Elsevier Science
Volume :	12
Issue/season :	4
Pages :	252-260
Audience :	International
ISSN :	1089-8603
City :	San Diego
Keywords :	[en] catalysis ; copper ; nitration ; nitrosation ; peroxynitrite ; propofol
Abstract :	[en] We report here on the role of copper (II) salts on the acceleration of peroxynitrite (ONOO-) decomposition and ONOO- reaction with the anaesthetic agent propofol (2,6-diisopropylphenol) in alkaline medium. We observed a strong acceleration of the ONOO- decomposition in alkaline medium in the presence of copper (I and II) salts. After 18 h of ONOO- reaction with propofol, we observed nitrosated, nitrated, and oxidized (quinone and diphenylquinone) derivatives of propofol, but in the presence of Cu(II) (20% molar vs ONOO-), the yields of quinone and nitrosopropofol strongly increased. We also observed that the temperature and the atmosphere influenced the effects of Cu(II) on ONOO- reactions with propofol: low temperatures promoted nitrosation and high temperatures promoted oxidation; O-2 atmosphere increased the general reactivity and the yield of nitrated and oxidized products. We highlighted the influence of Cu(II) salts on the radical character of the reaction by direct EPR technique. The exact mechanism of the Cu(II) catalysis remains unexplained, but we suggest the formation of a copper complex with propofol or, more probably, the oxidation of ONOO- into ONOO• by copper ions promoting the formation of quinone and nitrosopropofol according to a previously reported mechanism [M. Cudic, C. Ducrocq, Transformations of 2,6-diisopropylphenol by NO-derived nitrogen oxides, particularly peroxyrutrite, Nitric Oxide 4 (2000) 147-156]. © 2005 Elsevier Inc. All rights reserved.
Permalink :	http://hdl.handle.net/2268/940
DOI :	10.1016/j.niox.2005.03.003

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	428b.pdf		Publisher postprint	791.13 kB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5