Reference : Vaccine against human papillomavirus (HPV)-associated lesions induces collaboration betw...
Scientific congresses and symposiums : Poster
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/93729
Vaccine against human papillomavirus (HPV)-associated lesions induces collaboration between natural killer and dendritic cells in vitro.
English
Langers, Inge mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Reschner, Anca mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Renoux, Virginie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Dortu, Estelle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
BONIVER, Jacques mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
DELVENNE, Philippe mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Jacobs, Nathalie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Dec-2010
Yes
International
Annual Congress: New frontiers in dendritic cell biology
16-17/12/2010
Club Francophone des cellules dendritiques
Paris
France
[en] Cervical cancer, the second most frequent gynaecological malignancy in the world, is caused by infection with high-risk human papillomaviruses (HPV). HPV16 and/or 18 are detected in more than 70% of these tumours. Prophylactic HPV-L1 virus like particle (VLP) vaccines are highly efficient to protect against HPV16 and HPV18 infection, but not against established infection. In this context, we study the effect of HPV-VLP on natural killer cells (NK) and on the crosstalk between NK and Dendritic Cells (DC).
In order to know if HPV-VLP are able to enter in NK cells, we used fluorescent HPV-VLP with flow cytometry and confocal microscopy. HPV-VLP were internalised more rapidly in NK cells than in DC. They were already detected inside NK cells after 10 min of contact at 37°C. We also observed in CD107 assays, that HPV-VLP induce degranulation of NK cytotoxic granules.
Previous works have shown that HPV-VLP were able to activate DC. We confirmed these results and observed an increase of CD69 cell surface expression and IFN-γ production by NK cells in the presence of DC activated by VLP. Interestingly, NK cells seemed to further activate DC in the presence of VLP as shown by an up-regulation of HLA-DR and CD86 on DC. Moreover, NK cells in the presence of HPV-VLP induced the production of IL12p70, but not the immunosuppressive cytokine IL10.
Our results suggest that NK cells could play a role in the activation of DC induced by HPV-VLP during the vaccination against cervical cancer.
Supported by the Belgian FNRS-Télévie
http://hdl.handle.net/2268/93729

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