ORBi: Hody Stéphanie - Human muscle proteome modifications after acute or repeated eccentric exercises

Reference : Human muscle proteome modifications after acute or repeated eccentric exercises


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Laboratory medicine & medical technology Human health sciences : Orthopedics, rehabilitation & sports medicine Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/93697

Title :	Human muscle proteome modifications after acute or repeated eccentric exercises
Language :	English
Author, co-author :	Hody, Stéphanie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-Neuroscience > >]
	Leprince, Pierre [Université de Liège - ULg > > GIGA - Neurosciences >]
	Sergeant, K. [> > > >]
	Renaut, J. [Université de Liège - ULg > Département des sciences de la motricité > Kinésithérapie générale et réadaptation]
	Croisier, Jean-Louis [Université de Liège - ULg > Département des Sciences de la Motricité > > >]
	WANG, François-Charles [Centre Hospitalier Universitaire de Liège - CHU > > Médecine de l'appareil locomoteur >]
	Rogister, Bernard [Université de Liège - ULg > GIGA-Neuroscience > > >]
Publication date :	Dec-2011
Journal title :	Medicine & Science in Sports & Exercise
Publisher :	Lippincott Williams & Wilkins
Volume :	43
Issue/season :	12
Pages :	2281-2296
Audience :	International
ISSN :	0195-9131
e-ISSN :	1530-0315
City :	Hagerstown
Country :	MD
Abstract :	[en] INTRODUCTION:: DOMS (Delayed-Onset Muscle Soreness), a condition triggered by eccentric exercise, affects muscle cells at a biochemical level in a poorly understood fashion. The objective of the present study was to examine human muscle proteome modifications induced by strenuous eccentric exercises following a specific training aimed to prevent DOMS. METHODS:: Biopsy of the rectus femoris were taken from healthy human volunteers in three successive conditions: (1) at rest, (2) 24 hours after an injuring exercise protocol consisting of 3 series of 30 maximal contractions of the quadriceps on an isokinetic dynamometer, (3) 24 hours after a similar exercise bout preceded either by 5 eccentric training sessions, or no training. RESULTS:: Muscle damage was assessed before and 1 day after each maximal eccentric test by comparing three indirect markers: plasma activity of creatine kinase (CK), muscle stiffness and subjective pain intensity. Compared to the first eccentric test, those markers were reduced after the second test and further reduced if this second test followed the eccentric training, thus confirming the protective effect of such training. Muscle protein extracts were subjected to a 2D-DIGE proteomic analysis coupled with MALDI-TOF-MS protein identification. Surprisingly, we observed that myosin heavy chains decreased after the first eccentric test, and were reduced further with other contractile proteins after the second test. Furthermore, the expression of several glycolytic enzymes decreased only after the second test that was preceded by a specific training. CONCLUSION:: These findings suggest that the eccentric training resulted in a switch to oxidative metabolism, which may be associated with protection from DOMS.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/93697
DOI :	10.1249/MSS.0b013e318222edf3

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	Published ahead of print.pdf		Publisher postprint	4.54 MB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5