Reference : The Cell Wall Peptidoglycan of Bacillus megaterium KM. I. Studies on the Stereochemis...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/93330
The Cell Wall Peptidoglycan of Bacillus megaterium KM. I. Studies on the Stereochemistry of α,α'-Diaminopimelic Acid
English
Bricas, E. [> > > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > > > Service de Bactériologie > >]
Dezelee, P. [> > > >]
1-Aug-1967
Biochemistry
American Chemical Society
6
8
2598-2607
Yes (verified by ORBi)
International
0006-2960
1520-4995
Washington
DC
[en] amino acid sequence ; bacillus ; cell wall ; chromatography ; electrophoresis ; peptide hydrolases ; peptides ; pimelic acids ; polysaccharides ; spectrum analysis ; streptomyces ; enzymology ; cytology ; analysis ; metabolism
[en] α,α'-Diaminopimelic acid (DAP) occurs in the wall peptidoglycan of Bacillus megaterium KM predominantly in the form of its meso isomer (about 85% of the total residues) and, in minor amounts, in the form of its DD isomer. The amino groups on the L carbon of the meso-DAP residues are involved in peptide linkages to the glutamic acid residues. Most of the amino groups on the D carbon of the meso-DAP residues are free; some of them are substituted, thus probably serving to cross-link peptide subunits. These amino groups can be liberated by a Streptomyces endopeptidase. None of the DD-DAP residues have amino groups free. Moreover, these groups are not liberated by endopeptidase treatment. The peptidoglycan upon enzymatic degradation yields mainly two fractions. A major fraction is composed of disaccharide peptide monomer subunits containing only the meso isomer of DAP. A second minor fraction is composed of disaccharide peptide oligomers containing both meso and DD isomers of DAP. The meso-DAP residues isolated as monodinitrophenyl derivatives from both fractions have optical rotations and optical rotatory dispersions identical with that of synthetic monodinitrophenyl-meso-DAP obtained by dinitrophenylation of the amino group on the D carbon. The assignment of the DD configuration to the DAP residues which are not meso rests upon the optical rotatory properties of their bisdinitrophenyl derivatives.
Researchers ; Professionals
http://hdl.handle.net/2268/93330

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