Reference : Fully Automated Preparation and Conjugation of N-Succinimidyl 4-[(18)F]Fluorobenzoate ([...
Scientific journals : Article
Human health sciences : Radiology, nuclear medicine & imaging
http://hdl.handle.net/2268/93092
Fully Automated Preparation and Conjugation of N-Succinimidyl 4-[(18)F]Fluorobenzoate ([ (18)F]SFB) with RGD Peptide Using a GE FASTlab Synthesizer.
English
Thonon, David mailto [Université de Liège - ULg > > Centre de recherches du cyclotron]
Goblet, D. [> > > >]
Goukens, Eve mailto [Université de Liège - ULg > > Centre de recherches du cyclotron]
Kaisin, Geoffroy mailto [Université de Liège - ULg > > Centre de recherches du cyclotron]
Paris, Jérôme mailto [Université de Liège - ULg > > Centre de recherches du cyclotron]
Aerts, Joël mailto [Université de Liège - ULg > > Centre de recherches du cyclotron]
Lignon, S. [> > > >]
Franci, X. [> > > >]
Hustinx, Roland mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie organique de synthèse]
Luxen, André mailto [ > > ]
2011
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging
Yes (verified by ORBi)
International
1536-1632
1860-2002
[en] PURPOSE: The aim of this work was to automate the radiosynthesis of [(18)F]SFB, a widely used reagent for the labeling of biomolecules with (18)F on a new generation commercial synthesis module (FASTLab, GE Healthcare). PROCEDURES: Two synthesis approaches were implemented on this module: the classical "two-pot radiosynthesis" and the more recently described "one-pot" method. RESULTS: The "two-pot" approach affords [(18)F]SFB with a 42% decay-corrected yield in 57 min (n = 24) with a chemical purity sufficient to avoid an intermediate HPLC purification. The recently established "one-pot" method, afforded a product with a lower chemical purity, in the conditions used in this report. The lower d.c. yield obtained (32% (n = 15)) was related to the low (18)F labeling yields obtained in MeCN compared with DMSO. The subsequent conjugation step with a RGD (PRGD2) peptide was also successfully automated. CONCLUSIONS: The formulated [(18)F]FPRGD2 was obtained without any operator manipulation with a d.c. yield of 13% +/- 3% (n = 13) in 130 min, a radiochemical purity >98% and a specific activity of 140 +/- 40 TBq/mmol.
http://hdl.handle.net/2268/93092
10.1007/s11307-011-0470-x

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