Article (Scientific journals)
Improved vitrification method allowing direct transfer of goat embryos
Guignot, F.; Bouttier, A.; Baril, G. et al.
2006In Theriogenology, 66 (4), p. 1004-1011
Peer Reviewed verified by ORBi
 

Files


Full Text
GuignotF_Therio_2006.pdf
Publisher postprint (146.15 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
in vivo goat embryo; vitrification; slow freezing; transfer; embryo survival
Abstract :
[en] The aim of this study was to design a vitrification method suited to field embryo transfer experiments in goat. In a first experiment, a standard vitrification protocol, previously designed for sheep embryos was compared to slow freezing of goat embryos. No significant difference was observed on kidding rate (48% versus 69%, respectively), nor on embryo survival rate (35% versus 45%). Second experiment: all embryos were vitrified. After warming, embryos were either transferred directly (direct transfer), or after in vitro dilution of the cryoprotectants (conventional transfer). The kidding rate was not affected by the transfer method (38% versus 23%, respectively). However, embryo survival rate tended to be higher after direct transfer (26% versus 14%). Third experiment: OPS vitrification was compared to standard vitrification. The kidding rate was not affected (22% versus 39%, respectively), but the embryo survival rate was lower after OPS (14% versus 28%). Fourth experiment: 0.4 M sucrose was added with cryoprotectants in vitrification. The kidding rate after direct transfer was significantly enhanced after addition of sucrose (56% versus 27%, respectively), whereas embryo survival rate was not significantly affected (32% versus 18%). Fifth experiment: vitrification with sucrose supplementation was compared to slow freezing. No significant difference was observed after direct transfer on kidding rate (52% versus 31%, respectively), but embryo survival rate tended to be higher after vitrification (34% versus 2 1 %). In conclusion, our results indicate that addition of 0.4 M sucrose in association with direct transfer improves significantly the viability of goat vitrified embryos.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Guignot, F.
Bouttier, A.
Baril, G.
Salvetti, P.
Pignon, P.
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Touze, J. L.
Cognié, J.
Traldi, A. S.
Cognié, Yves
Mermillod, P.
Language :
English
Title :
Improved vitrification method allowing direct transfer of goat embryos
Publication date :
2006
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, New York, United States - New York
Volume :
66
Issue :
4
Pages :
1004-1011
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 March 2009

Statistics


Number of views
73 (2 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
40
Scopus citations®
without self-citations
32
OpenCitations
 
33

Bibliography


Similar publications



Contact ORBi