Reference : Pretreatments and enzymatic hydrolysis of Miscanthus x giganteus for oligosaccharides pr...

	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Engineering, computing & technology : Materials science & engineering
	To cite this reference:	http://hdl.handle.net/2268/93066

Title :	Pretreatments and enzymatic hydrolysis of Miscanthus x giganteus for oligosaccharides production: delignification degree and characterisation of the hydrolysis products
Language :	English
Author, co-author :	Vanderghem, Caroline [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Jacquet, Nicolas [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Blecker, Christophe [Université de Liège - ULg > Chimie et bio-industries > Technologie des industries agro-alimentaires >]
	Paquot, Michel [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Publication date :	4-Feb-2010
Audience :	International
Event name :	COST FP0901 meeting “Current needs in Biorefinery analytics”
Event date :	Du 4 au 5 février 2010
Event place (city) :	Vienne
Event country :	Autriche
Keywords :	[en] delignification ; miscanthus ; hydrolysis
Abstract :	[en] The aim of the present study is to compare two delignification methods (formic/acetic acid1 and soaking in aqueous ammonia) on Miscanthus x giganteus and to assess the suitability to produce cellobiose and other oligosaccharides after enzymatic hydrolysis. Oligosaccharides have recently gotten attention for their health benefits. Two methods were compared in order to quantify lignin: the acid detergent lignin method (procedure of Van Soest most commonly employed by animal scientist and agronomists for analysis of forages) and the Klason lignin procedure. Lignin concentrations in raw material determined by both methods were different; Klason lignin value (23.5%) was greater than the acid detergent lignin concentration (12.9%). Possible reasons of these results will be discussed. Pretreatment by the formic/acid mixture showed a better deliginification rate compared to the soaking in aqueous ammonia method. Results were based on Klason lignin. Analysis of the structural carbohydrates revealed that untreated miscanthus was mainly composed of glucose and xylose. Extracted pulps by both delignification methods were hydrolysed by commercial cellulases and hemicellulases. A major challenge is the characterisation of complex mixtures of lignocellulosic hydrolysates. In this study, the hydrolysis products were separated and quantified by highperformance anion exchange chromatography with pulsed amperometric detection (HPAECPAD). This method was successfully applied to the quantitative analysis of monosaccharides (glucose and xylose) and disaccharides (cellobiose and xylobiose) formed by the enzymatic hydrolysis of pretreated miscanthus. The influence of the pretreatments on the oligosaccharides yields will be presented.
Name of the research project :	TECHNOSE, LIGNOFUEL
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/93066

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