Reference : Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/9288
Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests
English
Karen, A. [> > > >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Sulon, Joseph [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Melo de Sousa, Noelita mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Szabados, K. [> > > >]
Reczigel, J. [> > > >]
Szenci, O. [> > > >]
May-2003
Theriogenology
Elsevier
59
9
1941-1948
Yes (verified by ORBi)
International
0093-691X
New York
NY
[en] pregnancy diagnosis ; P4 ; PAG ; ewe
[en] The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 Awassi x Merino ewes and blood samples were collected at Days 0 (day of the insemination), 18, 22, 29, 36, and 50 after artificial insemination (AI). Plasma P4 concentrations at Days 0 and 18 were determined by double antibody radioimmunoassay, while PAG concentrations at Days 22, 29, 36 and 50 were determined by a heterologous, double-antibody radioimmunoassay (RIA) using the bovine PAG 67 kDa subunit as tracer and standard and rabbit antiserum raised against a mixture of caprine 55 and 59 kDa PAG subunits as the first antibody. The discriminatory value for diagnosis of pregnancy by the P4 and the PAG-RIA tests was greater than or equal to1 ng/ml. Based on lambing data, the accuracy for diagnosing pregnant (sensitivity) and non-pregnant ewes (specificity) and predictivity of both tests were calculated. The sensitivity, specificity, positive and negative predictive values for P4 and PAG tests were 100, 95.4, 81.6, and 100% at Day 18 (P4) and 93.5, 100, 100 and 98.7% at Day 22 (PAG), respectively. For diagnosis of non-pregnant ewes the PAG test had significantly higher specificity than the P4 test (P < 0.05). It is concluded that ovine pregnancy can be reliably diagnosed at Day 22 after AI by using a heterologous radioimmunoassay of PAG.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/9288

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