Reference : Isolation and partial characterization of three pregnancy-associated glycoproteins fr...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/9283
Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta
English
El Amiri, B. [Université de Liège - ULG > > > > > >]
Remy, Benoit [ > > ]
Melo de Sousa, Noelita mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Joris, Bernard mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Otthiers, Nicole mailto [Université de Liège - ULg > Département des sciences de la vie > Labo de biochimie >]
Perenyi, Z. [> > > >]
Mboko, H. B. [> > > >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
2003
Molecular Reproduction and Development
Wiley Liss, Inc.
64
2
199-206
Yes (verified by ORBi)
International
1040-452X
1098-2795
New York
NY
[en] binucleate cell ; sheep ; pregnancy ; N-terminal microsequencing ; trophoblast
[en] Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal cotyledons and characterized biochemically. The isolation procedure included acid and ammonium sulfate precipitations and anion and cation exchange chromatographies. The isolated PAGs have different NH2-terminal amino acid sequences (RGSXLTILPLRNMRDIVY, ISRVSXLTIHPLRNIMDML, and RGSNLTIHPLRNIRD) and apparent molecular masses (55, 57, and 59 kDa). Each shows several isoforms with different pl values. The three proteins share high sequence identity with each other and with other ovine, bovine, and caprine PAGs. They have not been described previously. The ovPAG-59 sequence differs from the previously identified ovPAG-4 sequence (determined by DNA cloning and sequencing) at only one position among the 15 N-terminal residues. The newly characterized ovPAGs and the procedure used to isolate them will be helpful in producing new antisera for investigating PAG secretion in pregnant ewes.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/9283

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
ElAmiriB_MolReprodDev_2003.pdfPublisher postprint1.1 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.