Article (Scientific journals)
Chorionic expression of heterogeneous products of the PAG (Pregnancy-Associated Glycoprotein) gene family secreted in vitro throughout embryonic and foetal development in the pig
Szafranska, B.; Panasiewicz, G.; Majewska, M. et al.
2003In Reproduction Nutrition Development, 43 (6), p. 497-516
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Keywords :
chorionic glycoproteins; placenta; pPAG gene family; pig; pregnancy; trophectoderm
Abstract :
[en] Porcine PAG (pPAG) are placental products of a multigene family that is strongly expressed in the chorionic epithelium (trophoblast and trophectoderm). The objective of this study was to define a pattern of the pPAG proteins, secreted in vitro by chorionic explants harvested on 16-77 days of pregnancy. Trophoblastic and trophectodermal explants were collected from pregnant (PR) gilts (n = 27) and used for protein in vitro production (8-261 h). Endometrial explants of luteal-phase gilts (E10, n = 4) and pseudopregnant gilts (PsE, n = 2) were used as negative controls for protein immunoblotting. Proteins (PR, E10, PsE) were isolated mainly from incubation media, fractionated, dialysed and separated by SDS-PAGE. Heterogeneous Western blotting with various polyclonal anti-PAG sera raised against bovine or ovine antigens (anti-bPAG, or anti-oPAG) initially identified the pPAG proteins. Such blotting of fractionated chorionic proteins allowed for the isolation of porcine antigens that were employed as immunogens to raise several homologous antisera (anti-pPAG). Crude antisera were adsorbed on endometrial extracts or proteins of non-PR pigs, to remove non-relevant antibodies. The patterns of pPAG proteins secreted in Vitro varied throughout pregnancy (35-72 kDa). During implantation, similar to43 kDa (Day 16) or similar to68.1 kDa (Days 17-25) pPAG proteins were detected. During placentation and as pregnancy advanced (Days 31-77), similar to72.3 kDa pPAG proteins were observed. The secretions of parallel multiple smaller proteins (35.4-47.2 kDa), presumably, as forms of processed pPAG precursors, increased with the progress of gestation. In conclusion, the pPAG protein family plays a very important role during implantation, placenta formation and embryonic/foetal development in the pig.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Szafranska, B.
Panasiewicz, G.
Majewska, M.
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Chorionic expression of heterogeneous products of the PAG (Pregnancy-Associated Glycoprotein) gene family secreted in vitro throughout embryonic and foetal development in the pig
Publication date :
2003
Journal title :
Reproduction Nutrition Development
ISSN :
0926-5287
eISSN :
1297-9708
Publisher :
EDP Sciences, Les Ulis, France
Volume :
43
Issue :
6
Pages :
497-516
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 March 2009

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