ORBi: Berndt Sarah - Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium

Reference : Angiogenic activity of human chorionic gonadotropin through LH receptor activation on en...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/9262

Title :	Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium
Language :	English
Author, co-author :	Berndt, Sarah [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
	PERRIER d'HAUTERIVE, Sophie [Université de Liège - ULg > > Gynécologie-Obstétrique CHR >]
	Blacher, Silvia [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
	Pequeux, Christel [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
	Lorquet, Sophie [Université de Liège - ULg > > > Doct. sc. bioméd. & pharma. (Bologne) >]
	Munaut, Carine [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
	Applanat, Martinne [> > > >]
	Herve, M. A. [> > > >]
	Lamande, N. [> > > >]
	Corvol, P. [> > > >]
	van den Brule, Frederic [> > > >]
	Frankenne, Francis [> > > >]
	Poutanen, M. [> > > >]
	Huhtaniemi, I. [> > > >]
	Geenen, Vincent [Université de Liège - ULg > > Centre d'immunologie >]
	Noël, Agnès [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
	Foidart, Jean-Michel [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement]
Publication date :	Dec-2006
Journal title :	FASEB Journal
Publisher :	Federation of American Society for Experimental Biology
Volume :	20
Issue/season :	14
Pages :	2630-2632
Audience :	International
ISSN :	0892-6638
e-ISSN :	1530-6860
City :	Bethesda
Country :	MD
Keywords :	[en] angiogenesis ; hCG
Abstract :	[en] Successful embryo development requires an extensive endometrial angiogenesis in proximity of implantation site. The glycoprotein hCG is produced even before implantation by trophoblast in normal pregnancy. In this manuscript, we demonstrate an angiogenic effect of hCG in several in vivo (chick chorioallantoic membrane, matrigel plug assay, aortic ring assay) and in vitro experimental models. In contrast, human placental lactogen (hPL) did not display angiogenic properties. LH/hCG receptor was detected in endothelial cells by reverse-transcriptase polymerase chain reaction (RT-PCR) and by Western blotting. In mice aortic ring assay, angiostimulation by hCG was abrogated by deletion of LH/hCG receptor (LuRKO mice). Use of recombinant hCG and anti-hCG antibody (Ab) further confirmed the specificity of this angiogenic activity. By using dibutyryl cAMP, adenylate cyclase, or protein kinase A inhibitors, we demonstrate that hCG-mediated angiogenesis involves adenylyl-cyclase-protein kinase A activation. Addition of hCG to endometrial epithelial epithelial cells, but not to cultured endothelial cells, stimulated vascular endothelial growth factor (VEGF). VEGF and hCG also displayed additive activities. Altogether, these data demonstrate that peritrophoblastic angiostimulation may result from a paracrine dialogue between trophoblast, epithelial, and endothelial cells through hCG and VEGF.
Permalink :	http://hdl.handle.net/2268/9262
DOI :	10.1096/fj.06-5885fje

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