Article (Scientific journals)
Aspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins
Perenyi, Z. S.; Szenci, O.; Drion, Pierre et al.
2002In Reproduction in Domestic Animals, 37 (6), p. 324-329
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Keywords :
Aspartic proteinase; Radioimmunoassay; Pregnancy-associated glycoprotein; Pregnancy; Specificity
Abstract :
[en] Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml - 1 mg/ml). Pepsinogen cross-reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 microg/ml and 500 microg/ml concentrations, respectively. In the presence of pepsin, cross-reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 microg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross-react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross-reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Perenyi, Z. S.;  Université de Liège - ULiège
Szenci, O.
Drion, Pierre ;  Université de Liège - ULiège > Services généraux de l'Université
Banga-Mboko, H.;  Université de Liège - ULiège
Melo de Sousa, Noelita ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
El Amiri, Bouchra;  Université de Liège - ULiège
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Aspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins
Publication date :
2002
Journal title :
Reproduction in Domestic Animals
ISSN :
0936-6768
eISSN :
1439-0531
Publisher :
Blackwell Publishing, Berlin, Germany
Volume :
37
Issue :
6
Pages :
324-329
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 March 2009

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