Article (Scientific journals)
Characterization of pregnancy-associated glycoproteins extracted from zebu (Bos indicus) placentas removed at different gestational periods
Melo de Sousa, Noelita; Remy, Benoit; El Amiri, Bouchra et al.
2002In Reproduction Nutrition Development, 42 (3), p. 1-15
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Keywords :
pregnancy-associated glycoprotein; purification; zebu; placenta; N-terminal microsequencing
Abstract :
[en] In the present work, two biochemical approaches were used to characterize PAGs isolated from Bos indicus fetal cotyledons removed at different gestational ages. The first procedure included acidic and ammonium sulfate precipitations, anion and cation exchange chromatographies and the second included pepstatin-agarose affinity chromatography. A bovine PAG radioimmunoassay was used to monitor the immunoreactivity throughout the isolation procedures. The most immunoreactive fractions issued from cation exchange and affinity chromatographies were analyzed by SDS-PAGE and Western blotting, before transfer to a polyvinylidene difluoride (PVDF) membrane for NH2-microsequence determination. Use SDS-PAGE and Western blotting, different isoforms of PAG with apparent molecular masses of 51 to 69 kDa and isoelectric points varying from 4.4 to 6.7 were identified in the placentas from different gestational ages. N-terminal microsequencing (10 to 25 aa long) indicates the expression of one single terminal amino acid sequence in the Bos indicus placenta, which is 100% identical to the bovine PAG-1.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Melo de Sousa, Noelita ;  Université de Liège - ULiège
Remy, Benoit;  Université de Liège - ULiège
El Amiri, Bouchra
De Figueiredo, J. R.
Banga-Mboko, Henri
Goncalves, P. B. D.
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Characterization of pregnancy-associated glycoproteins extracted from zebu (Bos indicus) placentas removed at different gestational periods
Publication date :
2002
Journal title :
Reproduction Nutrition Development
ISSN :
0926-5287
eISSN :
1297-9708
Publisher :
EDP Sciences, Les Ulis, France
Volume :
42
Issue :
3
Pages :
1-15
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
CAPES - Coordenação de Aperfeicoamento de Pessoal de Nível Superior [BR]
Available on ORBi :
since 19 March 2009

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