[en] In prostate cancer therapy, as the components of the cell death pathway play an important role, it is conceivable that targeted transfer of apoptosis inducer genes into prostatic cells could be useful. A new gene, CIPAR1 (Castration Induced Prostatic Apoptosis Related gene 1) was identified by differential screening for genes that were upregulated when rat prostates were induced to undergo apoptosis. To define the role of CIPAR1 in the apoptosis pathway, we have therefore investigated its ability to induce apoptosis in the Mat LyLu cells. The CIPAR1 gene was transiently overexpressed in these cells. The rate of apoptosis in the transfected cells was estimated with the help of the following tests: qualitative methods (DNA ladder, TUNEL on slide) and also quantitative methods using flow cytolmetric analysis. Induction of apoptosis was higher in the pcDNA3-CIPAR1 transfected cells than in controls. The establishment of a stable cell line that contains the gene CIPAR1 under the control of an inducible promoter would be an useful model to study the role of CIPAR1 in prostatic cell death.
Research center :
Department of Biology (Area 13), University of York
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Cornet, Anne ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Overexpression and function of the CIPAR1 gene in a rat prostatic cell line.
