Reference : Spatio-temporal localization of the glial fibrillary acidic protein (GFAP) in the spiral...
Scientific congresses and symposiums : Poster
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/92060
Spatio-temporal localization of the glial fibrillary acidic protein (GFAP) in the spiral ganglion from the 16th embryonic day until the 25th postnatal day in rats
English
Cloes, Marie mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Thelen, Nicolas mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Johnen, Nicolas mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
31-Jan-2011
No
National
GIGA-Day
31 janvier 2011
GIGA-R, ULg
Liège
Belgique
[en] spiral ganglion ; GFAP
[en] The spiral ganglion (SG) is responsible for the conduction of the information between the sensory epithelium of the auditory organ (the organ of Corti) and the central nervous system. The origin and nature of the SG glial cells in mammals are barely known, although glial cells are essential to the development and the working of the nervous system. Using confocal microscopy, we studied the spatio-temporal distribution of the GFAP in the rat SG from the 16th embryonic day (E16) until the 25th postnatal day (P25). We performed a double-labelling experiment targeting GFAP- and betaIIItubulin-positive cells. BetaIII-tubulin is used for the labelling of (pro)neural cells, according to a previous preliminary study from our team. Our first results show clearly that the GFAP is expressed in the SG from P0 until P25. A homogenous labelling is found in the cytoplasm of a few dispersed unidentified cells among the (pro)neurons, whereas a granular labelling appears among a group of cells neighbouring the bundle of fibers innervating the organ of Corti. The identity of the GFAP-positive cells will be further investigated by electron microscopy. The reason why the labelling of the GFAP adopts those two different aspects is still unknown. Moreover, it seems that, surprisingly, some cells of the ganglion are not labelled by either marker. The possibility that such cells correspond to fibroblasts will be tested thanks to the labelling of vimentin.
Giga-Neurosciences
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Origine et caractérisation des cellules gliales du ganglion spiralé au cours du développement chez les mammifères
Researchers ; Professionals
http://hdl.handle.net/2268/92060

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
GIGA Day Poster Cloes_Jan2011.pdfAuthor postprint1.67 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.