Reference : Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/91729
Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases
English
Pollock, J. J. [New York University School > Department of Microbiology > > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Linder, R. [New York University School > Department of Microbiology > > >]
Salton, M. R. J. [New York University School > Department of Microbiology > > >]
Perkins, Harold R. [National Institute for Medical Research > > > > > > >]
Nieto, Manuel [National Institute for Medical Research > > > > >]
Leyh-Bouille, Mélinda [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Frère, Jean-Marie mailto [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Johnson, K. [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
1-Mar-1972
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
69
3
662-666
Yes (verified by ORBi)
International
0027-8424
1091-6490
Washington
DC
[en] alanine metabolism ; carbon isotopes ; carboxypeptidases antagonists & inhibitors ; electrophoresis ; glycine metabolism ; lysine metabolism ; penicillin g ; peptide hydrolases ; peptides analysis metabolism ; pimelic acids metabolism ; streptomyces enzymology ; tritium ; pharmacology
[en] In the presence of N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-Ala as donor, and either D-[(14)C]alanine, [(14)C]-glycine, or meso-[(3)H]diaminopimelic acid as acceptor, the DD carboxypeptidases from Streptomyces R61 and R39 catalyze a transpeptidation reaction with the release of terminal D-alanine from the donor and the formation of either N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-[(14)C]Ala, N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-[(14)C] Gly, or N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-meso- [(3)H]diaminopimelic acid. The reaction appears to be a true transpeptidation, and is not simply a "reversal of hydrolysis". Transpeptidation is inhibited by pencillin at concentrations that inhibit hydrolysis (carboxypeptidase action) of the donor peptide. There are differences in the specificity profiles of the Streptomyces enzymes for acceptor molecules:only the R61 enzyme used [(14)C]Gly-Gly as acceptor; transfer of N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala to this acceptor resulted in the formation of N(alpha),N(epsilon)-diacetyl-Lys-D-Ala-[(14)C] Gly-Gly, with the synthesis of a (D-Ala-Gly) peptide bond in an endoposition.
National Science Fondation ; Fonds de la Recherche Fondamentale Collective - FRFC
Researchers ; Professionals
http://hdl.handle.net/2268/91729
http://www.pnas.org/content/69/3/662.full.pdf+html

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