Article (Scientific journals)
Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases
Pollock, J. J.; Ghuysen, Jean-Marie; Linder, R. et al.
1972In Proceedings of the National Academy of Sciences of the United States of America, 69 (3), p. 662-666
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Keywords :
alanine metabolism; carbon isotopes; carboxypeptidases antagonists & inhibitors; electrophoresis; glycine metabolism; lysine metabolism; penicillin g; peptide hydrolases; peptides analysis metabolism; pimelic acids metabolism; streptomyces enzymology; tritium; pharmacology
Abstract :
[en] In the presence of N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-Ala as donor, and either D-[(14)C]alanine, [(14)C]-glycine, or meso-[(3)H]diaminopimelic acid as acceptor, the DD carboxypeptidases from Streptomyces R61 and R39 catalyze a transpeptidation reaction with the release of terminal D-alanine from the donor and the formation of either N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-[(14)C]Ala, N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-[(14)C] Gly, or N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-meso- [(3)H]diaminopimelic acid. The reaction appears to be a true transpeptidation, and is not simply a "reversal of hydrolysis". Transpeptidation is inhibited by pencillin at concentrations that inhibit hydrolysis (carboxypeptidase action) of the donor peptide. There are differences in the specificity profiles of the Streptomyces enzymes for acceptor molecules:only the R61 enzyme used [(14)C]Gly-Gly as acceptor; transfer of N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala to this acceptor resulted in the formation of N(alpha),N(epsilon)-diacetyl-Lys-D-Ala-[(14)C] Gly-Gly, with the synthesis of a (D-Ala-Gly) peptide bond in an endoposition.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Pollock, J. J.;  New York University School > Department of Microbiology
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Linder, R.;  New York University School > Department of Microbiology
Salton, M. R. J.;  New York University School > Department of Microbiology
Perkins, Harold R.;  National Institute for Medical Research
Nieto, Manuel;  National Institute for Medical Research
Leyh-Bouille, Mélinda;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Frère, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Johnson, K.;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Language :
English
Title :
Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases
Publication date :
01 March 1972
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
ISSN :
0027-8424
eISSN :
1091-6490
Publisher :
National Academy of Sciences, Washington, United States - District of Columbia
Volume :
69
Issue :
3
Pages :
662-666
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NSF - National Science Foundation [US-VA]
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
Available on ORBi :
since 26 May 2011

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