Article (Scientific journals)
Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein
Joris, Bernard; Ledent, P.; Kobayashi, T. et al.
1990In FEMS Microbiology Letters, 58 (1), p. 107-113
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Keywords :
beta-lactamase induction; Escherichia coli; Bacillus Licheniformis; BLAR-CTD
Abstract :
[en] A cloning vector has been constructed which allows production and export by Escherichia coli of the Met346-Arg601 carboxy terminal domain of the 601 amino acid BLAR sensory-transducer involved in beta-lactamase inducibility in Bacillus licheniformis. The polypeptide, referred to as BLAR-CTD, accumulates in the periplasm of E. coli in the form of a water-soluble, Mr 26,000 penicillin-binding protein. These data and homology searches suggest that BLAR has a membrane topology similar to that of other sensory-transducers involved in chemotaxis.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Joris, Bernard ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Ledent, P.;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Kobayashi, T.;  Waksman Institute of Microbiology
Lampen, J.-O.;  Waksman Institute of Microbiology
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein
Publication date :
15 June 1990
Journal title :
FEMS Microbiology Letters
ISSN :
0378-1097
eISSN :
1574-6968
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
58
Issue :
1
Pages :
107-113
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
Fond de la Recherche de la Faculté de Médecine ULg
National Institutes for Allergy and Infectious Deseases
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