[en] A cloning vector has been constructed which allows production and export by Escherichia coli of the Met346-Arg601 carboxy terminal domain of the 601 amino acid BLAR sensory-transducer involved in beta-lactamase inducibility in Bacillus licheniformis. The polypeptide, referred to as BLAR-CTD, accumulates in the periplasm of E. coli in the form of a water-soluble, Mr 26,000 penicillin-binding protein. These data and homology searches suggest that BLAR has a membrane topology similar to that of other sensory-transducers involved in chemotaxis.
Centre d'ingénierie des protéines - CIP
Fonds de la Recherche Scientifique Médicale - FRSM ; Fond de la Recherche de la Faculté de Médecine ULg ; National Institutes for Allergy and Infectious Deseases