Reference : Dual Multimodular Class a Penicillin-Binding Proteins in Mycobacterium Leprae
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/90990
Dual Multimodular Class a Penicillin-Binding Proteins in Mycobacterium Leprae
English
Lepage, Sophie [Université de Liège - ULg > Institut de Chimie > Centre d'Ingénierie des Protéines > > >]
Dubois, Philippe mailto [Université de Liège - ULg > Institut de Chimie > Centre d'Ingénierie des Protéines > > >]
Ghosh, Thushar-Kanti [Université de Liège - ULg > Institut de Cimie > Centre d'Ingénierie des Protéines > > >]
Joris, Bernard mailto [Université de Liège - ULg > > Centre d'Ingénierie des Protéines > > >]
Mahapatra, Sebabrata [Bose Institute-Calcutta > Department of Chemistry > > >]
Kundu, Manikuntula [Bose Institute-Calcutta > Department of Chemistry > > >]
Basu, Joyoti [Bose Institute-Calcutta > Department of Chemistry > > >]
Chakrabarti, Parul [Bose Institute-Calcutta > Department of Chemistry > > >]
Cole, Stewart-T [Institut Pasteur (Paris) > > Unite de Génétique Moléculaire Bactérienne > >]
Distèche, Martine mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Institut de Chimie > Centre d'ingénierie des Protéines > > >]
Jul-1997
Journal of Bacteriology
American Society for Microbiology (ASM)
179
14
4627-4630
Yes (verified by ORBi)
International
0021-9193
1098-5530
Washington
DC
[en] Escherichia coli/genetics ; Penicillin-Binding Proteins
[en] The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
Centre d'Ingénierie des Protéines - CIP
Fonds de la Recherche Scientifique Médicale - FRSM
Researchers ; Professionals
http://hdl.handle.net/2268/90990

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