Secreted metalloprotease gene family of Microsporum canis

[en] Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes.

Disciplines :

Immunology & infectious disease

Author, co-author :

Brouta, Frédéric

Descamps, Frédéric

Monod, Michel

Vermout, Sandy

Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires

Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires

Language :

English

Title :

Secreted metalloprotease gene family of Microsporum canis

Publication date :

October 2002

Journal title :

Infection and Immunity

ISSN :

0019-9567

eISSN :

1098-5522

Publisher :

Amer Soc Microbiology, Washington, United States - Washington

Volume :

Issue :

Pages :

5676-5683

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 March 2009

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