Reference : Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisi...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/9071
Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis
English
Descamps, Frédéric mailto [> > > >]
Brouta, Frédéric [> > > >]
Vermout, Sandy mailto [> > > >]
Monod, Michel [> > > >]
Losson, Bertrand mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Mignon, Bernard mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
18-Aug-2003
FEMS Immunology & Medical Microbiology
Elsevier Science Bv
38
1
29-34
Yes (verified by ORBi)
International
0928-8244
Amsterdam
[en] keratinase ; subtilisin-like protease ; ELISA ; lymphoproliferation test ; Microsporum canis ; Pichia pastoris
[en] A secreted 31.5-kDa keratinolytic subtilase (SUB3; AJ431180) is thought to be a Microsporum canis virulence factor and represents a candidate for vaccination trials. In this study, the recombinant keratinase (r-SUB3) was produced by the Pichia pastoris expression system and purified to homogeneity. Recombinant SUB3 displayed identical biochemical properties with the native protease. Experimentally cutaneously infected guinea pigs showed specific lymphoproliferative response towards r-SUB3, while no specific humoral immune response was induced except for one animal. The heterologous expression of SUB3 provides a valuable tool for addressing further investigations on the role of this keratinase in the specific cellular immune response and on its use in vaccination trials in the cat. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/9071

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