Reference : Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secon...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/90622
Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.
English
Sabri, Ahmed mailto [Université de Liège - ULg > Centre Wallon de Biologie Industrielle (CWBI) > > >]
Leroy, Pascal mailto [Université de Liège - ULg > Department of Functional and Evolutionary Entomology > > >]
Haubruge, Eric mailto [Université de Liège - ULg > Department of Functional and Evolutionary Entomology > Vice-Recteur de Gembloux Agro Bio Tech > >]
Hance, Thierry [Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center > > >]
Frere, Isabelle [Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center > > >]
Destain, Jacqueline mailto [Université de Liège - ULg > Centre Wallon de Biologie Industrielle (CWBI) > > >]
Thonart, Philippe mailto [Université de Liège - ULg > Centre Wallon de Biologie Industrielle (CWBI) > > >]
2010
International Journal of Systematic and Evolutionary Microbiology
Society for General Microbiology
Yes (verified by ORBi)
International
1466-5026
1466-5034
Reading
United Kingdom
[en] Serratia symbiotica ; Aphids ; Symbionts ; Aphis fabae
[en] An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing 1% of each of following substrates; dextrose, yeast extract and peptone of casein. Pure culture was achieved through the use of solid-phase culture on the same medium and was designated CWBI-2.3T. 16S ribosomal DNA sequence analysis revealed that CWBI-2.3T strain was a member of the gamma-3 subgroup of the class Proteobacteria, having high sequence identity (>99%) with the R-Type of secondary endosymbiont found in several aphid species Serratia symbiotica. As strain CWBI-2.3T (=LMG 25624T =DSM 23270T) was the first R-type symbiont to be isolated and characterized, we proposed to designate it as the type strain of Serratia symbiotica species.
Centre Wallon de Biologie Industrielle
Région Wallonne de Belgique
SOLAPHID
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/90622
10.1099/ijs.0.024133-0
This work was supported by "la Région Wallonne de Belgique" convention Waleo2
253 n°061/6288.

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