ORBi: Sabri Ahmed - Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.

Reference : Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secon...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/90622

Title :	Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.
Language :	English
Author, co-author :	Sabri, Ahmed [Université de Liège - ULg > Centre Wallon de Biologie Industrielle (CWBI) > > >]
	Leroy, Pascal [Université de Liège - ULg > Department of Functional and Evolutionary Entomology > > >]
	Haubruge, Eric [Université de Liège - ULg > Department of Functional and Evolutionary Entomology > Vice-Recteur de Gembloux Agro Bio Tech > >]
	Hance, Thierry [Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center > > >]
	Frere, Isabelle [Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center > > >]
	Destain, Jacqueline [Université de Liège - ULg > Centre Wallon de Biologie Industrielle (CWBI) > > >]
	Thonart, Philippe [Université de Liège - ULg > Centre Wallon de Biologie Industrielle (CWBI) > > >]
Publication date :	2010
Journal title :	International Journal of Systematic and Evolutionary Microbiology
Publisher :	Society for General Microbiology
Audience :	International
ISSN :	1466-5026
e-ISSN :	1466-5034
City :	Reading
Country :	United Kingdom
Keywords :	[en] Serratia symbiotica ; Aphids ; Symbionts ; Aphis fabae
Abstract :	[en] An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing 1% of each of following substrates; dextrose, yeast extract and peptone of casein. Pure culture was achieved through the use of solid-phase culture on the same medium and was designated CWBI-2.3T. 16S ribosomal DNA sequence analysis revealed that CWBI-2.3T strain was a member of the gamma-3 subgroup of the class Proteobacteria, having high sequence identity (>99%) with the R-Type of secondary endosymbiont found in several aphid species Serratia symbiotica. As strain CWBI-2.3T (=LMG 25624T =DSM 23270T) was the first R-type symbiont to be isolated and characterized, we proposed to designate it as the type strain of Serratia symbiotica species.
Research units :	Centre Wallon de Biologie Industrielle
Funders :	Région Wallonne de Belgique
Name of the research project :	SOLAPHID
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/90622
DOI :	10.1099/ijs.0.024133-0
Commentary :	This work was supported by "la Région Wallonne de Belgique" convention Waleo2 253 n°061/6288.

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