Reference : Development of an in vitro endotoxin test for monoolein-water liquid crystalline gel ...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/89856
Development of an in vitro endotoxin test for monoolein-water liquid crystalline gel for use as an implant
English
Ouédraogo, Moustapha mailto [UFR - Sciences de la Santé, Université de Ouagadougou (Burkina Faso) > > > >]
Semdé, Rasmané [UFR - Sciences de la Santé, Université de Ouagadougou (Burkina Faso) > > > >]
Somé, Issa T. [UFR - Sciences de la Santé, Université de Ouagadougou (Burkina Faso) > > > >]
Ouédraogo, Moussa [UFR - Sciences de la Santé, Université de Ouagadougou, Burkina Faso > > > >]
Ouédraogo, Rasmata [UFR - Sciences de la Santé, Université de Ouagadougou, Burkina Faso > > > >]
Henschel, Viviane [Université Libre de Bruxelles - ULB > Pharmacy Institute > > >]
Evrard, Brigitte mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Dubois, Jacques [Université Libre de Bruxelles - ULB > Pharmacy Institute > > >]
Amighi, Karim [Université Libre de Bruxelles - ULB > Pharmacy Institute > > >]
Guissou, Innocent P. [UFR - Sciences de la Santé, Université de Ouagadougou, Burkina Faso > > > >]
Dec-2009
Tropical Journal of Pharmaceutical Research [=TJPR]
8
6
501-508
Yes (verified by ORBi)
International
1596-5996
[en] in vitro bacterial endotoxin test ; liquid crystalline gels ; test validation ; monoolein-water
[en] Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a monoolein-water liquid crystalline gel as a model. Methods : The gel-clot technique was used. The gel was dissolved first in isopropyl myristate, and the endotoxins were extracted with water for bacterial endotoxin test. Tests for the labelled lysate sensitivity and interfering factors were performed to validate the developed method. The limit of detection of endotoxin in the gel was also determined. Results : The labelled lysate sensitivity was confirmed. It was not influenced by the presence of extracts from the gels. Endotoxins in the contaminated test gels were completely extracted. Endotoxin concentration in the tested gels was below the calculated threshold endotoxin level. Conclusion : A method to perform in vitro endotoxins test of liquid crystalline gels was successfully developed and validated. Application of the technique to gels currently being developed in our laboratories indicate that the gels were apyrogenic.
http://hdl.handle.net/2268/89856

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